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The Effect of Tethering on the Chemical Kinetics of Single Bonds

Published online by Cambridge University Press:  26 February 2011

Melanie Nguyen-Duong ,
Karl-Wilhelm Koch  and
Rudolf Merkel
Melanie Nguyen-Duong
Affiliation:
m.duong@fz-juelich.de, Research Center Jülich, ISG-4, Germany
Karl-Wilhelm Koch
Affiliation:
karl.w.koch@uni-oldenburg.de, Carl von Ossietzky University Oldenburg, Faculty V, Biochemistry, Germany
Rudolf Merkel
Affiliation:
r.merkel@fz-juelich.de, Research Center Jülich, ISG-4, Germany
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Abstract

We investigated the force induced dissociation of single specific bonds between protein A and immunoglobulin G. In these experiments both proteins were tethered to microbeads. Pi-conewton forces were applied by means of an ultrasoft spring. Upon bond formation the thermal fluctuations of this soft spring were reduced. This resulted in a sizeable contribution to the free enthalpy of bonding. Including this effect we calculated the relation between the lifetime of bonds connecting both microbeads and the lifetime of the bond between free molecules. We found that the lifetime depended on the stiffness of the force probe. Moreover, the lifetime was reduced by almost two orders of magnitude. Both predictions were verified in our experiments.

Keywords

biologicaladhesionstrength
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 899: Symposium N – Dynamics in Small Confining Systems VIII , 2005 , 0899-N03-01
Copyright
Copyright © Materials Research Society 2006

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References

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