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DNA Electrophoresis on Two-dimensional Arrays of SiO2 Beads

Published online by Cambridge University Press:  17 March 2011

Young-Soo Seo ,
V.A. Samuilov ,
J. Sokolov ,
M. Rafailovich ,
D. Gersappe  and
B. Chu
Young-Soo Seo
Affiliation:
Department of Materials Science and Engineering
V.A. Samuilov
Affiliation:
Department of Materials Science and Engineering
J. Sokolov
Affiliation:
Department of Materials Science and Engineering
M. Rafailovich
Affiliation:
Department of Materials Science and Engineering
D. Gersappe
Affiliation:
Department of Materials Science and Engineering
B. Chu
Affiliation:
Department of Chemistry, SUNY at Stony Brook, NY 11794
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Abstract

A novel approach of fabricating 2-D arrays of SiO2 beads on a Si surface using the Langmuir-Blodgett (LB) technique is reported. The corrugated surfaces were tested as a separation media for surface electrophoresis of DNA molecules. The measured electrophoretic mobility for λ-DNA is only 20% slower than previously measured on a flat Si wafer. This indicates that the separation mechanism is due to surface friction rather than biased reptation as reported by Tinland in the three dimensional Silica bead matrix where the mobility is two orders of magnitude smaller.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 636: Symposium D – Nonlithographic and Lithographic Methods of Nanofabrication-From Ultralarge Scale , 2000 , D3.7.1
Copyright
Copyright © Materials Research Society 2001

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