Hostname: page-component-76fb5796d-zzh7m Total loading time: 0 Render date: 2024-04-26T06:25:36.096Z Has data issue: false hasContentIssue false
  • English
  • Français

Demonstration of biocompatibility of single walled carbon nanotubes with blood leukocytes

Published online by Cambridge University Press:  07 March 2012

Krishnakiran Medepalli ,
Bruce Alphenaar ,
Ashok Raj  and
Palaniappan Sethu
Krishnakiran Medepalli
Affiliation:
Department of Electrical and Computer Engineering, Speed School of Engineering, Louisville, KY
Bruce Alphenaar
Affiliation:
Department of Electrical and Computer Engineering, Speed School of Engineering, Louisville, KY
Ashok Raj
Affiliation:
Division of Pediatrics, School of Medicine, Louisville, KY
Palaniappan Sethu
Affiliation:
Department of Bioengineering, Speed School of Engineering, University of Louisville, Louisville, KY
Get access

Abstract

Single walled carbon nanotubes (SWNTs) possess unique structural and functional properties. Their ability to be functionalized with different biomolecules makes them excellent candidates for biomedical applications like targeted drug delivery and cancer diagnostics. However, prior to use in therapeutic applications, biocompatibility of SWNTs needs to be thoroughly investigated. Blood is a living tissue and contains cells which can potentially interact with SWNTs during the drug delivery process. The interaction of leukocytes in blood with the SWNTs can provide information regarding the immune response of the host to the nanotubes. Here, we evaluated the acute immune response of leukocytes in blood to SWNTs via (a) direct interaction, due to the presence of SWNTs in circulation and (b) indirect interaction, due to the presentation of SWNTs to leukocytes via antigen presenting cells. These SWNTs were non-covalently functionalized with single stranded DNA (ss-DNA) that acts as a surfactant for suspending SWNTs in aqueous solutions and also serves as a backbone for attaching and transporting different biomolecules. Isolation of cells from blood was done using density gradient centrifugation. Early activation markers were used to study the activation of different leukocyte subpopulations and any activation results in changes of these markers. Flow cytometry was done to analyze the different subpopulations. Results of our study demonstrated that ss-DNA functionalized SWNTs do not elicit an immune response from leukocytes in blood via direct or indirect interaction. This intensive study demonstrates the biocompatibility of single walled carbon nanotubes and paves the way for their safe use in drug delivery and cancer therapeutics without cytotoxicity.

Keywords

biomedicalnanoscaleoptical properties
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1416: Symposium JJ – Nanofunctional Materials, Nanostructures and Nanodevices for Cancer Applications , 2012 , mrsf11-1416-jj05-20
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Nie, S., Xing, Y., Kim, G. J. and Simons, J. W., Annual Review of Biomedical Engineering, 9(1), 257, (2007).Google Scholar
2. Klumpp, C., Kostarelos, K., Prato, M. and Bianco, A.. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1758(3), 404 (2006).Google Scholar
3. Hu, H., Ni, Y., Montana, V., Haddon, R. C. and Parpura, V.. Nano Letters, 4(3), 507 (2004).Google Scholar
4. Teker, K., Sirdeshmukh, R., Sivakumar, K., Lu, S., Wickstrom, E. and Wang, H.N., et al. . Nanobiotechnology, 1(2), 171, (2005).Google Scholar
5. Wong Shi Kam, N. and Dai, H., Phys Status Solidi B Basic Res, 243, 3561, (2006),Google Scholar
6. Cherukuri, P., Bachilo, S.M., Litovsky, S.H. and Weisman, R.B., J Am Chem Soc, 126, 15638, (2004).Google Scholar
7. Monteiro-Riviere, N.A., Nemanich, R.J., Inman, A.O., Wang, Y.Y. and Riviere, J.E., Toxicol Lett, 155, 377, (2005).Google Scholar
8. Pulskamp, K., Diabaté, S. and Krug, H.F., Toxicol Lett, 168, 58, (2007).Google Scholar
9. Davoren, M., Herzog, E., Casey, A., Cottineau, B., Chambers, G. and Byrne, H.J, et al. ., Toxicol In Vitro, 21, 438, (2007).Google Scholar
10. Dumortier, H., Lacotte, S., Pastorin, G., Marega, R., Wu, W. and Bonifazi, D., et al. ., Nano Lett, 6, 1522, (2006).Google Scholar
11. Bai, , , Y. Zhang, , , J. Zhang, , , Q. Mu, , , W. Zhang, and Butch, E.R., et al. ., Nat Nano, 5, 683, (2010) [10.1038/nnano.2010.153]Google Scholar
12. Muller, J., Huaux, F., Moreau, N., Misson, P., Heilier, J.F. and Delos, M., et al. ., Toxicol Appl Pharmacol, 207, 221, (2005).Google Scholar
13. Zhou, L.J. and Tedder, T.F.., Proc Natl Acad Sci U S A, 93, 2588 (1996).Google Scholar
14. Sethu, P., Moldawer, L.L., Mindrinos, M.N., Scumpia, P.O., Tannahill, C.L. and Wilhelmy, J., et al. ., Anal Chem, 78, 5423, (2006).Google Scholar
15. Russom, A., Sethu, P., Irimia, D., Mindrinos, M.N., Calvano, S.E. and Garcia, I., et al. ., Clin Chem, 54, 891, (2008).Google Scholar
16. Gea, Cuicui, Dua, Jiangfeng, Zhaoa, Lina, Wanga, Liming, Liua, Ying, Lia, Denghua, Yanga, Yanlian, Zhoub, Ruhong, Zhaoa, Yuliang, Chaid, Zhifang, and Chena, Chunying, PNAS, 108(41),(2011).Google Scholar