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Contribution of Collagen, Mineral and Water Phases to the Nanomechanical Properties of Bone

Published online by Cambridge University Press:  01 February 2011

Amanpreet K. Bembey ,
Vanessa Koonjul ,
Andrew J. Bushby ,
Virginia L. Ferguson  and
Alan Boyde
Amanpreet K. Bembey
Affiliation:
Department of Materials, Queen Mary, University of London, London, UK.
Vanessa Koonjul
Affiliation:
Department of Materials, Queen Mary, University of London, London, UK.
Andrew J. Bushby
Affiliation:
Department of Materials, Queen Mary, University of London, London, UK.
Virginia L. Ferguson
Affiliation:
Department of Materials, Queen Mary, University of London, London, UK. BioServe Space Technologies, Department of Aerospace Engineering Sciences, University of Colorado, Boulder, CO, USA.
Alan Boyde
Affiliation:
Dental Biophysics, Queen Mary, University of London, London, UK.
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Abstract

Cortical bone is an anisotropic material, and its mechanical properties are determined by its composition as well as its microstructure. Mechanical properties of bone are a consequence of the proportions of, and the interactions between, mineral, collagen and water. Mid-shaft palmar cortical tissue from the equine third metacarpal bone is relatively dense and uniform with low porosity. The mainly primary osteons are aligned to within a few degrees of the long axis of the bone. Beams of compact cortical bone were prepared to examine effects of dehydration and embedding and to study contribution of collagen and mineral to nano-scale material properties. Five beams were tested: untreated (hydrated); 100% ethanol (dehydrated); or embedded in poly-methylmethacrylate (PMMA) for one normal, one decalcified, and one deproteinated bone sample. Elastic modulus was obtained by nanoindentation using spherical indenters, with the loading direction transverse [1] and longitudinal to the bone axis. By selectively removing water, mineral and organic components from the composite, insights into the ultrastructure of the tissue can be gained from the corresponding changes in the experimentally determined elastic moduli.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 844: Symposium Y – Mechanical Properties of Bio-Inspired and Biological Materials , 2004 , Y1.8/R2.8
Copyright
Copyright © Materials Research Society 2005

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References

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