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Comparison of Single and Double Stranded Dna Binding to Polypyrrole.

Published online by Cambridge University Press:  15 February 2011

Rajiv Pande ,
Jeong - Ok Lim ,
Kenneth A. Marx ,
Sukant K. Tripathy  and
David L. Kaplan
Rajiv Pande
Affiliation:
Center for Advanced Materials and Department of Chemistry, University of Massachusetts Lowell, Lowell, MA 01854,
Jeong - Ok Lim
Affiliation:
Center for Advanced Materials and Department of Chemistry, University of Massachusetts Lowell, Lowell, MA 01854,
Kenneth A. Marx
Affiliation:
Center for Advanced Materials and Department of Chemistry, University of Massachusetts Lowell, Lowell, MA 01854,
Sukant K. Tripathy
Affiliation:
Center for Advanced Materials and Department of Chemistry, University of Massachusetts Lowell, Lowell, MA 01854,
David L. Kaplan
Affiliation:
Biotechnology Division, U.S. Army Natick Research, Development & Engineering Center, Natick, MA 01760.
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Abstract

The polycation conducting polymer, oxidized polypyrrole (PPy), possesses the ability to form complexes with DNA. Our previously proposed diffusion limited binding model for double helical DNA was also found to be applicable to single stranded DNA in this study. Single stranded DNA was found to bind PPy at a nearly identical level to that of double helical DNA. An investigation of electropolymerized PPy film morphology using SEM revealed two distinctly differing surface morphologies for the Platinum (Pt) electrode face (smooth) and polymeric growth face (rough). The DNA uptake levels were found to be consistently different on either surface, being higher on the rough surface. DNA penetrated into the disk interior with increasing time periods of exposure while a similar phenomenon but to a lesser extent was observed for single stranded DNA.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 292: Symposium S – Biomolecular Materials , 1992 , 135
Copyright
Copyright © Materials Research Society 1993

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