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Chitosan-alginate Hybrid Scaffolds for in Vitro Bone Tissue Regeneration

Published online by Cambridge University Press:  01 February 2011

Zhensheng Li ,
Hassna R. Ramay ,
Kip D. Hauch  and
Miqin Zhang
Zhensheng Li
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
Hassna R. Ramay
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
Kip D. Hauch
Affiliation:
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Miqin Zhang
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
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Abstract

This paper reports the development of a biodegradable porous scaffold made from naturally derived chitosan and alginate polymers for bone tissue engineering. The scaffold has a 3-D interconnected porous structure and was fabricated through thermally induced phase separation. The mechanical test showed that the scaffold has compressive strength of 0.46 ± 0.02 MPa — about 4 times that of the pure chitosan scaffold. The cell-material interaction study indicated that osteoblast cells seeded on the chitosan-alginate scaffold attach and proliferate well. The mineral deposition occurred after 3 days of culture and formed large chunks after 7 days. The chitosan-alginate scaffold was also found to have desirable swelling property and was structurally stable in solutions of different pH. The chitosan-alginate scaffold can be prepared from solutions of neutral pH allowing growth factors to be incorporated homogeneously into the scaffold for sustained release. This research demonstrated a technique by which a polymer-based biodegradable scaffold can be made to have high porosity up to 92% and excellent mechanical and biological properties.

Keywords

chitosanalginatescaffoldbone tissuebiodegradable
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 873: Symposium K – Biological and Bio-Inspired Materials and Devices , 2005 , K8.2
Copyright
Copyright © Materials Research Society 2005

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