Hostname: page-component-77c89778f8-gvh9x Total loading time: 0 Render date: 2024-07-18T23:42:49.832Z Has data issue: false hasContentIssue false
  • English
  • Français

Cationic Gelatin as a Gene Carrier

Published online by Cambridge University Press:  21 February 2011

K. E. Brown ,
J. Bathon ,
C. H. Huang ,
R. Dalai  and
K. W. Leong
K. E. Brown
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
J. Bathon
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
C. H. Huang
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
R. Dalai
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
K. W. Leong
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
Get access

Abstract

Cationic gelatin was evaluated as a non-viral vector for cell transfection. We hypothesized that cationic gelatin would be a nontoxic alternative to already existing viral and non-viral cationic vectors. Cationic gelatin was synthesized by modifying gelatin with hexanediamine. Complexation of cationic gelatin with psv-β-gal plasmid caused an electrophoretic mobility shift of the plasmid. Cationic gelatin/DNA complexes were optimized in terms of transfection efficiency in CHODUK XB1 and COS 7 cell lines. Maximal gene expression for both cell types occurred in serum free medium with chloroquine (100 μM) at cationic gelatin/DNA ratios of approximately 2 and 7. In comparison with DEAE dextran, polylysine and Lipofectamine, cationic gelatin was the most efficient in transfecting COS 7 cells, with up to 18% cells transfected. In a dye reduction cytotoxicity assay, cationic gelatin caused < 5% of cells to become nonviable at a concentration of 100 μg/ml, while the other transfection reagents tested at the same concentration caused 25–100% of cell death. These results suggest that cationic gelatin holds promise as an effective vehicle for gene delivery to mammalian cells.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 394: Symposium Z – Polymers in Medicine and Pharmacy , 1995 , 61
Copyright
Copyright © Materials Research Society 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Behr, J., Acc. Chem. Res., 26,274–278 (1993).Google Scholar
2. Cotten, m. and Wagner, E., Current Opinion in Biotechnology,4,705–710 (1993).Google Scholar
3. Haensler, J., and Szoka, F.C., Bioconjugate Chem.,4, 372–379 (1993).Google Scholar
4. Cotten, M., Wagner, E., and Birnstiel, M.L., Methods in Enzymology, 289, 618–644 (1993).Google Scholar