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Calcium Phosphate Bioceramics with Tailored Crystallographic Texture for Controlling Cell Adhesion

Published online by Cambridge University Press:  01 February 2011

Hyunbin Kim ,
Renato P. Camata ,
Sukbin Lee ,
Gregory S. Rohrer ,
Anthony D. Rollett ,
Kristin M. Hennessy ,
Susan L. Bellis  and
Yogesh K. Vohra
Hyunbin Kim
Affiliation:
bin1031@uab.edu, University of Alabama at Birmingham, Department of Materials Science and Engineering, Birmingham, AL, 35294, United States
Renato P. Camata
Affiliation:
camata@uab.edu, University of Alabama at Birmingham, Department of Physics, Birmingham, AL, 35294, United States
Sukbin Lee
Affiliation:
sukbin@andrew.cmu.edu, Carnegie Mellon University, Department of Materials Science and Engineering, Pittsburgh, PA, 15213, United States
Gregory S. Rohrer
Affiliation:
gr20@andrew.cmu.edu, Carnegie Mellon University, Department of Materials Science and Engineering, Pittsburgh, PA, 15213, United States
Anthony D. Rollett
Affiliation:
rollett@andrew.cmu.edu, Carnegie Mellon University, Department of Materials Science and Engineering, Pittsburgh, PA, 15213, United States
Kristin M. Hennessy
Affiliation:
khennes3@uab.edu, University of Alabama at Birmingham, Department of Physiology and Biophysics, Birmingham, AL, 35294, United States
Susan L. Bellis
Affiliation:
bellis@uab.edu, University of Alabama at Birmingham, Department of Physiology and Biophysics, Birmingham, AL, 35294, United States
Yogesh K. Vohra
Affiliation:
ykvohra@uab.edu, University of Alabama at Birmingham, Department of Physics, Birmingham, AL, 35294, United States
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Abstract

The orientation distribution of crystalline grains in calcium phosphate coatings produced by pulsed laser deposition was investigated using an X-ray pole-figure diffractometer. Increased laser energy density of a KrF excimer laser in the 4–7 J/cm2 range leads to the formation of hydroxyapatite grains with the c-axis preferentially aligned perpendicularly to the substrates. This preferred orientation is most pronounced when the plume direction of incidence is normal to the substrate. This crystallographic texture of hydroxyapatite grains in the coatings is associated with the highly directional and energetic nature of the ablation plume. Anisotropic stresses, transport of hydroxyl groups, and dehydroxylation effects during deposition all seem to play important roles in texture development. Studies of cell adsorption using human Mesenchymal stem cells reveal that hydroxyapatite coatings with strong texture and random orientation show different cell adsorption behavior, which is consistent with the notion that protein attach differently on different faces of hydroxyapatite crystals. Cells seeded on textured coatings exhibit better spreading and adhesion compared to those placed on randomly oriented coatings.

Keywords

ablationCabiomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 925: Symposium BB – Mechanotransduction and Engineered Cell-Surface Interactions , 2006 , 0925-BB02-07
Copyright
Copyright © Materials Research Society 2006

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