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An Introduction to Liposome Encapsulation of Bioactive Polyanions

Published online by Cambridge University Press:  26 February 2011

Raphael M. Ottenbrite  and
Juno Sunamoto
Raphael M. Ottenbrite
Affiliation:
Department of Chemistry and Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23284
Juno Sunamoto
Affiliation:
Laboratory for Artificial Cell Technology, Nagasaki University, Nagasaki, Japan
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Abstract

Polyanionic polymers with differing molecular weight, lipophilicity, and charge density were synthesized and their ability to non-specifically “activate” macrophages was evaluated. The level of activation of test polymer-elicited macrophages was monitored by their tumoricidal capacity. In addition, the ability of test polymers to inhibit growth of Lewis lung in vivo was evaluated. We have determined that the activation of macrophages by test polymers differs from that of the conventional activating agents pyran and C. parvum.

We then found that these polycarboxylic acid copolymers could be encapsulated by liposomes and administered intravenously. Superoxide production by murine peritoneal exudate macrophages activated by the test polyanions encapsulated in liposomes was increased by 300% in the first 2 hours compared to macrophages activated by the free polymers.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 110: Symposium M – Biomedical Materials and Devices , 1987 , 401
Copyright
Copyright © Materials Research Society 1988

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