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AN ENZYMATIC METHOD TO OBTAIN A NEW SCAFFOLD FOR ENGINEERING CARTILAGE

Published online by Cambridge University Press:  15 February 2013

David M. Giraldo Gomez ,
Fernando Villegas Alvarez ,
David Garciadiego Cazares ,
Avelina Sotres Vega  and
Maria C. Piña Barba
David M. Giraldo Gomez
Affiliation:
Instituto de Investigaciones en Materiales, UNAM, Ciudad Universitaria, Circuito Exterior s/n, C.P. 04510, México D.F., México.
Fernando Villegas Alvarez
Affiliation:
Departamento de Cirugía-Facultad de Medicina, UNAM, Ciudad Universitaria, Circuito Exterior s/n, C.P. 04510, México D.F., México.
David Garciadiego Cazares
Affiliation:
Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación, Calzada México Xochimilco N° 289, C.P. 14389, México D.F., México.
Avelina Sotres Vega
Affiliation:
Departamento de Investigación en Cirugía Experimental, Instituto Nacional de Enfermedades Respiratorias “Ismael Cossio Villegas”, Calzada de Tlalpan N° 4502, C.P. 14080, México D.F., México
Maria C. Piña Barba
Affiliation:
Instituto de Investigaciones en Materiales, UNAM, Ciudad Universitaria, Circuito Exterior s/n, C.P. 04510, México D.F., México.
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Abstract

The purpose of this study was to achieve a descellularized scaffold from cartilage tissue, which can be used as xenograft for cartilage tissue regeneration.

This work presents the results obtained using one method to wash porcine trachea in order to remove cellular material from the extracellular matrix and to avoid the immune reaction using enzymatic detergent and partial enzymatic degradation with Deoxyribonuclease I (DNase-I), Ethylenediaminetetraacetic Acid (EDTA) and Trypsin. This treatment was qualitatively evaluated by Scanning Electron Microscopy (SEM), and H&E Stain (Histology), and quantitatively evaluated by DNA quantification. The thermal characterization of the descellularized scaffold was carried out using Termogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). The type of collagen obtained from the scaffold was determined through SDS-PAGE electrophoresis. When using Enzymatic Treatment (ET) to wash trachea tissue, it is possible to obtain an acellular xenograft; this procedure has the potential to avoid rejection reactions of the xenograft.

Keywords

biomimetic (assembly)biologicalbiomaterial
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 139 - 144
Copyright
Copyright © Materials Research Society 2013 

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