Hostname: page-component-76fb5796d-skm99 Total loading time: 0 Render date: 2024-04-26T13:09:24.966Z Has data issue: false hasContentIssue false
  • English
  • Français

Active Polymer Nanoparticles: Delivery of Antibiotics

Published online by Cambridge University Press:  01 February 2011

Monica Rabinovich ,
Shankari N. Somayaji ,
Rajeev Raghavan Pillai ,
Michael C. Hudson ,
J. Kent Ellington ,
Michael Bosse ,
James Horton  and
Kenneth E. Gonsalves
Monica Rabinovich
Affiliation:
mmrabino@email.uncc.edu, University of North Carolina at Charlotte, Polymer Nanotechnology Lab.at Center for Optoelectronics and Dept. of Chemistry, 9201 University City Blv., 358 GRIGG Hall, Charlotte, NC, 28223, United States, 704-687-8290, 704-687-8241
Shankari N. Somayaji
Affiliation:
ssomayaj@uncc.edu, University of North Carolina at Charlotte, Department of Biology, 9201 University City Blvd., 257 Woodward Hall, Charlotte, NC, 28223, United States
Rajeev Raghavan Pillai
Affiliation:
rraghav1@uncc.edu, University of North Carolina at Charlotte, Polymer Nanotechnology Lab.at Center for Optoelectronics and Dept. of Chemistry, 9201 University City Blvd., 358 GRIGG Hall, Charlotte, NC, 28223, United States
Michael C. Hudson
Affiliation:
mchudson@email.uncc.edu, University of North Carolina at Charlotte, Department of Biology, 9201 University City Blvd., 257 Woodward Hall, Charlotte, NC, 28223, United States
J. Kent Ellington
Affiliation:
jke@carolina.rr.com, Carolinas Medical Center, Department of Orthopaedic Surgery, 1616 Scott Ave., Charlotte, NC, 28203, United States
Michael Bosse
Affiliation:
Michael.Bosse@carolinashealthcare.org, Carolinas Medical Center, Department of Orthopaedic Surgery, 1616 Scott Ave., Charlotte, NC, 28203, United States
James Horton
Affiliation:
James.Horton@carolinashealthcare.org, Carolinas Medical Center, Department of Internal Medicine, 1000 Blythe Blvd., Charlotte, NC, 28232, United States
Kenneth E. Gonsalves
Affiliation:
kegonsal@email.uncc.edu, University of North Carolina at Charlotte, Polymer Nanotechnology Lab.at Center for Optoelectronics and Dept. of Chemistry, 9201 University City Blvd., 358 GRIGG Hall, Charlotte, NC, 28223, United States
Get access

Abstract

Antibiotic-encapsulated PLA and PLGA nanoparticles were prepared by the single emulsion-solvent evaporation technique. Different PLA and PLGA systems were prepared, varying the copolymer composition and the amount of the surfactant polyvinyl alcohol. Characterization and drug loading studies were performed by UV-Visible spectrophotometry, dynamic light scattering, and scanning electron microscopy (SEM).

Simultaneously, in order to model the diffusion of the nanoparticles within the osteoblast, QDs such as functionalized InGaP/ZnS and polymer encapsulated InGaP/ZnS nanoparticles were added to confluent cultures of primary mouse osteoblasts. Following PreFer fixation, cultures were examined via confocal microscopy. QDs were clearly visible within osteoblasts.

Keywords

nanoscalenanostructurepolymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1019: Symposium FF – Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease , 2007 , 1019-FF05-06
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Soppimath, K. S., Aminadhavi, T. M., Kulkarni, A. R., Rudzinski, W. E., “Biodegradable polymeric nanoparticles as drug delivery devices”, J. of Controlled Release, 70, 1–20 (2001).10.1016/S0168-3659(00)00339-4Google Scholar
2. Song, C. X., Labhasetwar, V., Murphy, H., Qu, X., Humphrey, W.R., Shebuski, R. J., Levy, R. J., “Formulation and characterization of biodegradable nanoparticles for intravascular local drug delivery”, J. of Controlled Release, 43, 197–212 (1997).10.1016/S0168-3659(96)01484-8Google Scholar
3. Calhoun, J. H., Mader, J. T., “Treatment of Osteomyelitis with a Biodegradable Antibiotic Implant”, Clinical Orthopaedics and Related Research, 341, 206–214 (1997).10.1097/00003086-199708000-00030Google Scholar
4. Mandal, T. K., Bostanian, L. A., Graves, R. A., Chapman, S. R., “poly(d,l-Lactide-CoGlycolide) Encapsulated Poly(Vinyl Alcohol) Hydrogel as a drug Delivery System”, Pharmaceutical Research, 19, 1713–1719 (2002).10.1023/A:1020765615379Google Scholar
5. Faria, T. J. de, Campos, A. Machacado de, Senna, E. Lemos, “Preparation and Characterization of Poly(D,L-Lactide )(PLA) and Poly(D,L-Lactide)-Poly(Ethylene Glycol) (PLA-PEG) Nanocapsules Containing Antitumoral Agent Methotrexate”, Macromol. Symp”, 229, 228–233 (2005).10.1002/masy.200551128Google Scholar
6. Lamprecht, A., Ubrich, N., Perez, M. Hombreiro, Lehr, C. M., Hoffman, M., Maaincent, P., “Biodegradable monodispersed nanoparticles prepared by pressure homogenizationemulsification”, International Journal of Pharmaceutics, 184, 97–105 (1999).Google Scholar
7. Astete, C. E., Sabliov, C. M., “Synthesis and Characterization of PLGA Nanoparticles”, J. Biomater. Sci. Polymer Edn., 17, 247–289 (2006).10.1163/156856206775997322Google Scholar
8. Hans, M. L., Lowman, A. M., “Biodegradable nanoparticles for drug delivery and targeting”, Solid State & Material Science, 6, 319–327 (2002).Google Scholar
9. Kumar, M. N. V. Ravi, Bakowsky, U., Lehr, C. M., “Preparation and Characterization of cationic PLGA Nanospheres as DNA carries”, Biomaterials, 25, 1771–1777 (2004).Google Scholar
10. Laurencin, C. T., Attawia, A. M., Lu, L. Q., Borden, M. D., Lu, H. H., Gorum, W. J., Lieberman, J. R., “Poly(lactide-co-glycolide)/hydroxyapatite delivery of BMP-2-producing cells: a regional gene therapy approach to bone regeneration”, Biomaterials, 22, 1271–1277 (2001).Google Scholar
11. Gonsalves, K. E., Jin, S., Baraton, M. I., “Synthesis and surface characterization of functionalized polylactide copolymer microparticles”, Biomaterials, 19, 1501–1505 (1998).Google Scholar
12. Kwon, H., Lee, J., Choi, S., Jang, Y., Kim, J., “Preparation of PLGA nanoparticles containing estrogen by emulsification-diffusion method”, Colloids and Susfaces, 182, 123–130 (2001).10.1016/S0927-7757(00)00825-6Google Scholar
13. Panyam, J., Labhasetwar, V., “Biodegradable nanoparticles for drug delivery to cells and tissue”, Advanced Drug Delivery Reviews, 55, 329–347 (2003).Google Scholar
14. Lagerholm, B. C., Wang, M., Ernst, L. A., Ly, D. H., Liu, H., Bruchez, M. P., Waggoner, A. S., “Multicolor Coding of Cells with Cationic Peptide Coated Quantum Dots”, Nanoletters, 4, 2019–2022 (2004).10.1021/nl049295vGoogle Scholar
15. Bruchez, M. P., Moronne, M., Gin, P., Weiss, S., Alivisatos, A. P., “Conjugation of Luminescent Quantum Dots with Antibodies Using an Engineered Adaptor Protein to Provide New Reagents for Fluoroimmunoassays”, Science, 281, 2013–2016 (1998).Google Scholar
16. Larson, D. R., Zipfel, W. R., Williams, R. M., Clark, S. W., Bruchez, M. P., Wise, F. W., Webb, W. W., “Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo”, Science, 300, 1434–1436 (2003).10.1126/science.1083780Google Scholar
17. Jaiswal, J. K., Mattoussi, H., Mauro, J. M., Simon, S. M., “Long-term multiple color imaging of live cells using quantum dot bioconjugates”, Nature Biotechnology, 21, 47–51 (2003).10.1038/nbt767Google Scholar
18. Rosenthal, S. J., Tomlinson, I., Adkins, E. M., Schroeter, S., Adams, S., Swafford, L., McBride, J., Wang, Y., DeFelice, L. J., Blakely, R. D., “Targeting Cell Surface Receptors with Ligand-Conjugated Nanocrystals”, J. Am. Chem. Soc., 124, 4586–4594 (2002).Google Scholar
19. Ellington, J. K., Harris, M., Webb, L., Smith, B., Smith, T., Tan, K., Hudson, M., “Intracellular Staphylococcus aureus. A mechanism for the indolence of Osteomyelitis”, J. Bone Joint Surg. Br. 85(6), 918–21 (2003).Google Scholar
20. Lucke, M., Schmidmaier, G., Sadoni, S., Wildemann, B., Schiller, R., Stemberger, A., Haas, N.P., Raschke, M.., “A new model of implant-related osteomyelitis in rats”. J. Biomed Mater. Res. B Appl. Biomater., 67(1), 593–602 (2003).10.1002/jbm.b.10051Google Scholar
21. Ellington, J. K., Reilly, S. S., Ramp, W. K., Smeltzer, M. S., Kellam, J. F., Hudson, M. C. “Mechanisms of Staphylococcus aureus invasion of cultured osteoblasts”, Microb. Pathog., 26(6), 317–23 (1999).Google Scholar
22. Alexander, E. H., Rivera, F. A., Marriot, I., Anguita, J., Bost, K. L., Hudson, M.C., “Staphylococcus aureus-induced tumor necrosis factor-related apoptosis-inducing ligand expression mediates apoptosis and caspase-8 activation in infected osteoblasts”. BMC Microbiol, 3, 5 (2003)Google Scholar
23. Varela, L. M., Garcia, M., Perez-Rodriguez, M., Taboada, P., Ruso, J. M., Mosquera, V.,“Multilayer adsorption model for the protein-ligand interaction”, Journal of Chemical Physics, 114(17), 7682–7687 (2001).Google Scholar
24. Zweers, M., Engbers, G., Grijpma, D., Feijen, J.,“Release of anty-restenosis drugs from poly(ethylene oxide)-poly(dl-lactic-co-glycolic acid) nanoparticles”, Journal of Controlled Release, 114(3), 317–324 (2006).10.1016/j.jconrel.2006.05.021Google Scholar