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Synthesis, Surface and Cell-Adhesion Properties of Polyurethanes Containing Covalently Grafted RGD-Peptides

Published online by Cambridge University Press:  15 February 2011

Horng-Ban Lin
Affiliation:
Meadox Medicals, Inc., 112 Bauer Drive, Oakland, NJ 07436
Stuart L. Cooper
Affiliation:
University of Delaware, College of Engineering, 135 DuPont Hall, Newark, DE 19716
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Abstract

In an attempt to improve endothelial cell adhesion and growth on a polyurethane copolymer, cell adhesive RGD-containing peptides were grafted to the polymer backbone. Two peptide grafting reaction schemes, including one-step and two-step approaches, were developed. Amino acid analysis confirmed that the two-step approach had a higher peptide coupling efficiency. The two-step reaction scheme was utilized to prepare GRGDSY, GRGDVY and GRGESY (inactive control) peptide grafted polyurethanes with two different peptide densities (100 and 250 μmol/g polymer). Dynamic contact angle measurements indicated that the surfaces of the peptide grafted polyurethanes were more hydrophilic than the starting and carboxylated versions of the precursor polyurethane. In-vitro endothelial cell adhesion experiments showed that, without the presence of serum in culture medium, the GRGDSY- and GRGDVY-grafted polyurethanes dramatically enhanced cell attachment and spreading. Increasing the peptide density from 100 to 250 μmol/g polymer for the GRGDSYand GRGDVY-grafted polyurethanes resulted in an increase in cell attachment. With approximately the same peptide density (100 or 250 μmol/g polymer), the GRGDVY-grafted polymers supported more adherent cells than the GRGDSY-grafted polymers. Similar trends were observed in the in-vitro endothelial cell growth studies using culture medium containing serum and endothelial cell growth supplement. These RGD-peptide grafted polyurethanes may be useful in providing an easily prepared cell-adhesive substrate for various implantable devices and hybrid organs.

Type
Research Article
Copyright
Copyright © Materials Research Society 1994

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References

1. Lelah, M.D. and Cooper, S.L., Polyurethanes in Medicine (CRC Press, Boca Raton, 1986), pp. 5771.Google Scholar
2. Ito, Y. and Imanishi, Y., Crit. Rev. Biocompat., 5, 45 (1989).Google Scholar
3. Gospodarowicz, D. and Ill, C., J. Clin. Invest., 65, 1351 (1980).CrossRefGoogle Scholar
4. Nichols, N.K., Gospodarowicz, D., Kessler, T.R., and Oslen, D.B., Trans. Am. Soc. Artif. Intern. Organs, 27, 208 (1981).Google Scholar
5. Massia, S.P. and Hubbell, J.A., J. Biomed. Mater. Res., 25, 223 (1991).CrossRefGoogle Scholar
6. Matsuda, T., Kondo, A., Makino, K., and Akutsu, T., Trans. Am. Soc. Artif. Intern. Organs, 35, 677 (1989).CrossRefGoogle Scholar
7. Lin, H.-B., Zhao, Z.-C., García-Echeverría, C., Rich, D.H., and Cooper, S.L., J. Biomater. Sci., Polymer Ed., 3, 217 (1992).CrossRefGoogle Scholar
8. Beck-Sickinger, A.G., Schnorrenberg, G., Metzger, J., and Jung, G., Int. J. Peptide Protein Res., 38, 25 (1991).CrossRefGoogle Scholar
9. King, D.S., Fields, C.G., and Fields, G.B., Int. J. Peptide Protein Res., 36, 255 (1990).CrossRefGoogle Scholar
10. Ohno, M., and Abe, T., J. Immunol. Methods, 145, 199 (1991).CrossRefGoogle Scholar
11. Hirano, Y., Kando, Y., Hoyashi, T., Goto, K., and Nakajima, A., J. Biomed. Mater. Res., 25, 1523 (1991).Google Scholar
12. Massia, S.P. and Hubbell, J.A., J. Cell Biol., 114, 1089 (1991).CrossRefGoogle Scholar
13. Dejana, E., Colella, S., Conforti, C., Abbadini, M., Gobili, M., and Marchisio, P.C., J. Cell Biol., 107, 1215 (1988).CrossRefGoogle Scholar
14. Fath, K., Edgell, C.-J. S., and Burridge, K., J. Cell Sci., 92, 67 (1989).Google Scholar
15. van Wachem, P.B., Hogt, A.H., Beugeling, T., Feigen, J., Bantjes, A., Detmers, J.P., and Aken, V. van, Biomaterials, 8, 323 (1987).CrossRefGoogle Scholar
16. Steele, J.G., Johnson, G., Norris, W.D., and Underwood, P.A., Biomaterials, 12, 531 (1991).CrossRefGoogle Scholar

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Synthesis, Surface and Cell-Adhesion Properties of Polyurethanes Containing Covalently Grafted RGD-Peptides
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