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A Portable Setup for Molecular Detection by Transmission LSPR

Published online by Cambridge University Press:  04 February 2013

Giulia Cappi ,
Enrico Accastelli ,
Fabio M. Spiga ,
Vera Cantale ,
Maria A. Rampi ,
Luca Benini  and
Carlotta Guiducci
Giulia Cappi*
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
Enrico Accastelli
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
Fabio M. Spiga
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
Vera Cantale
Affiliation:
Dipartimento di Chimica, Università di Ferrara, via Borsari 46, 44121 Ferrara, Italy.
Maria A. Rampi
Affiliation:
Dipartimento di Chimica, Università di Ferrara, via Borsari 46, 44121 Ferrara, Italy.
Luca Benini
Affiliation:
Dipartimento di Elettronica, Informatica e Sistemistica, Università di Bologna, Viale Risorgimento 2, 40136 Bologna, Italy.
Carlotta Guiducci
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
*
*Contact author’s e-mail: giulia.cappi@epfl.ch
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Abstract

In the framework of bioanalytics and multiple array detection, we developed a fully portable and low-cost detection system based on Localized Surface Plasmon Resonance (LSPR) in a transmission configuration (T-LSPR). The transmission approach is suitable to be scaled to small dimension systems and to enable high-density array measurements on the same platform. Our setup is made out of off-the-shelf components and consists of a set of discrete light sources and a couple of light-detectors which enable a differential measurement setup. An algorithm fits the measured data and extracts the information of the plasmon peak position in the spectrum.

The performance of our T-LSPR measurement system has been characterized on a set of Fluorinated Tin Oxide-coated glass slides covered with gold Nanoislands (NIs). The samples have been modified with a single-stranded DNA layer and a real-time DNA hybridization experiment has been performed. Here we demonstrate that the proposed T-LSPR device, based on the characterization of the plasmon peak with a differential approach, is able to monitor real-time DNA hybridization on surface, and to precisely measure the position of the peak with a standard deviation in wavelength of 0.2 nm.

Keywords

sensorbiologicaloptical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1479: Symposium S – Nanostructured Materials and Nanotechnology—2012 , 2012 , pp. 27 - 32
Copyright
Copyright © Materials Research Society 2012

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References

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