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Microparticle filtration using carbon nanotubes and impedance characterisation for gold microelectrodes sensor system

Published online by Cambridge University Press:  31 January 2011

Ashish Mathur
Affiliation:
mathur-a@email.ulster.ac.uk, University of Ulster, NIBEC, Belfast, Northern Ireland, United Kingdom
Eyad M Hamad
Affiliation:
e.hamad@ulster.ac.uk, University of Ulster, NIBEC, Belfast, Northern Ireland, United Kingdom
Subhadeep Mukhopadhyay
Affiliation:
smukh9@yahoo.com, University of Ulster, NIBEC, Belfast, Northern Ireland, United Kingdom
Susanta Sinha Roy
Affiliation:
s.sinha-roy@ulster.ac.uk
Jim A McLaughlin
Affiliation:
jad.mclaughlin@ulster.ac.uk, University of Ulster, NIBEC, Belfast, Northern Ireland, United Kingdom
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Abstract

Blood-cell-free serum is required for most clinical chemistry tests. At present bend micro channel and polymeric pillars are used in polymer based microfluidic devices (such as PMMA) for the blood filtration. In this study, we have fabricated carbon nanotube (CNT) pillars on silicon from 20-50 μm in diameter with ˜10 μm spacing and integrate them inside the microfluidic channel with a view of using these for blood plasma filtration from whole blood, with passive capillary flow. Our main objective is to design a novel sensor, comprising CNT arrays, to filter/control whole blood flow, with an integrated micro patterned gold electrode which will be sealed by bonding into microfluidics structures. We have characterized the microfluidic channel by measuring the meniscus movement profiles. Also gold inter-digitated electrodes (IDEs) were fabricated on glass and immobilized with an antibody. These IDEs were used as an impedance-based biosensor using label-free antigen – antibody interaction. At a fixed frequency, the IDEs gave a linear response across the range of concentrations of secondary antibodies investigated (0 to 500 μg/mL).

Type
Research Article
Copyright
Copyright © Materials Research Society 2010

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