Skip to main content Accessibility help
×
Home
Hostname: page-component-747cfc64b6-bv7lh Total loading time: 0.267 Render date: 2021-06-15T22:20:08.579Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true }

Cytotoxic and Anti-cancer Effects of Nickel Nanowires against Pancreatic Cancer Cells

Published online by Cambridge University Press:  30 March 2012

Md. Zakir Hossain
Affiliation:
Applied Biosciences Emphasis, Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Wisam J. Khudhayer
Affiliation:
Department of Systems Engineering, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Rozina Akter
Affiliation:
Applied Biosciences Emphasis, Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Tansel Karabacak
Affiliation:
Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Maurice G. Kleve
Affiliation:
Department of Biology, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Corresponding
Get access

Abstract

Cytotoxicity study of magnetic nanomaterials is a key consideration for biomedical applications. Very little is known about the cytotoxic and anti-cancer effects of nickel nanowires (Ni NWs) on mammalian cells and their interaction with proliferating cancer cells. Current therapeutics do not address the full heterogeneity of pancreatic cancers due to the resistance to apoptosis and does not suffice for a successful treatment. Therefore, synthesis of novel anticancer drugs continues to be a potential topic for pancreatic cancer research. In this study, we have investigated the cellular toxicity and anti-cancer effects of Ni NWs in one of the most aggressive human pancreatic ductal cancer (Panc-1) cell lines with the objective of development of a potential treatment strategy. Ni NWs were fabricated in a custom-made setup utilizing the electrodeposition method. Elemental analysis, crystallographic structure, and morphological properties of the synthesized Ni NWs were investigated using Energy Dispersive X-ray Analysis (EDAX), X-Ray Diffraction (X-RD) and Scanning Electron Microscopy (SEM), respectively. Panc-1 cell cultures were maintained according to a slightly modified American Type Culture Collection (ATCC) protocol. Morphological apoptogenic characteristics assessment of the Ni NWs induced Panc-1 cell was accomplished using phase contrast microscopy (PCM). Two commercially available cytotoxicity procedures including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and trypan blue (TB) assays were utilized to determine the qualitative and quantitative cytotoxicity and anti-cancer effects of Ni NWs. As a negative control, Panc-1 cells without Ni NWs treatment were used in all experiments. Phase contrast microscopy (PCM) was used to confirm the Ni NWs internalization by Panc-1 cells. Both the MTT and TB assays, qualitatively and quantitatively confirmed the cytotoxic and anti-cancer effects of Ni NWs treated Panc-1 cells in vitro in both concentration and exposure-time dependent manners. We studied the cytotoxic and anti-cancer effects of Ni NWs on Panc-1 cells using novel integrated bionanotechnological approaches to understand the corresponding biological pathway with the objective of developing pancreatic cancer treatment. More specifically, we explored the molecular mechanisms associated with the pathway involved in Ni NWs induced toxicity against Panc-1 cells. Our results demonstrated that Ni NWs show strong candidacy for targeting cell selective applications in pancreatic cancer therapy. Key words: Nickel Nanowires, anti-cancer effects, pancreatic cancer.

Type
Research Article
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below.

References

1. Lenhard, R.E.., Osteen, R. T. and Gansler, T., ACS. Clinical Oncology, American Cancer Society’s textbook of cancer. Wiley-Blackwell, USA (2000).Google Scholar
2. Ward, T. H., Cummings, J., Dean, E., Br J Cancer. 99: p. 841846 (2008).CrossRefGoogle Scholar
3. Tyler, D., American Journal of Gastroenterology, 96, p. 25322534 (2001).CrossRefGoogle Scholar
4. Choi, D., Fung, A., Moon, H., Ho, D., Chen, Y., Kan, E., Rheem, Y., Yoo, B., Myung, N. Biomed Microdevices. Volm. 9(2): p. 1438 (2007).CrossRefGoogle Scholar
5. Lu, H., Shi, X., Costa, M. and Huang, C., Molecular and Cellular Biochemistry. Volume 279, Numbers 1–2, 4567 (2005).CrossRefGoogle Scholar
6. Costa, M., Fresenius J. Anal Chem. 361: p. 381385 (1998).CrossRefGoogle Scholar
7. Jeong, J. H., Kim, S.H., Min, J. H., Kim, Y. K., and Kim, S.S., Physica status solidi (a), 204: p. 40254028 (2007).CrossRefGoogle Scholar
8. Potten, C. and Wilson, J., Apoptosis: The Life and Death of Cells. Cambridge University Press, USA (2005).Google Scholar
9. Hossain, M. Z. and Kleve, M.G., International Journal of Nanomedicine, vol. 6, p. 14751485 (2011).CrossRefGoogle Scholar
10. Mousavi, S. H., Tayarani, N. Z., and Parsaee, H., Cell Mol Neurobiol 30: 185191(2010).CrossRefGoogle Scholar
11. Zhang, X.D., Wu, J.J., Gillespie, S., Borrow, J., and Hersey, P., Clinical Cancer Research p. 13351364 (2006).Google Scholar
12. Barisic, K., Petrik, J., and Rumora, L., Acta Pharm. 53 p. 151164 (2003).Google Scholar
13. Hellebrand, E.E. and Varbiro, G., Drug Discoveries & Therapeutics. 4(2): p. 5461 (2010).Google Scholar

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Cytotoxic and Anti-cancer Effects of Nickel Nanowires against Pancreatic Cancer Cells
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Cytotoxic and Anti-cancer Effects of Nickel Nanowires against Pancreatic Cancer Cells
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Cytotoxic and Anti-cancer Effects of Nickel Nanowires against Pancreatic Cancer Cells
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *