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Controlling neuronal growth and connectivity via directed self-assembly of proteins

Published online by Cambridge University Press:  12 March 2013

Daniel Rizzo ,
Ross Beighley ,
James D. White  and
Cristian Staii
Daniel Rizzo
Affiliation:
Department of Physics and Astronomy, and Center for Nanoscopic Physics, Tufts University, 4 Colby Street, Medford, MA, 02155, U.S.A.
Ross Beighley
Affiliation:
Department of Physics and Astronomy, and Center for Nanoscopic Physics, Tufts University, 4 Colby Street, Medford, MA, 02155, U.S.A.
James D. White
Affiliation:
Department of Physics and Astronomy, and Center for Nanoscopic Physics, Tufts University, 4 Colby Street, Medford, MA, 02155, U.S.A.
Cristian Staii
Affiliation:
Department of Physics and Astronomy, and Center for Nanoscopic Physics, Tufts University, 4 Colby Street, Medford, MA, 02155, U.S.A.
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Abstract

Materials that offer the ability to influence tissue regeneration are of vital importance to the field of Tissue Engineering. Because valid 3-dimensional scaffolds for nerve tissue are still in development, advances with 2-dimensional surfaces in vitro are necessary to provide a complete understanding of controlling regeneration. Here we present a method for controlling nerve cell growth on Au electrodes using Atomic Force Microscopy -aided protein assembly. After coating a gold surface in a self-assembling monolayer of alkanethiols, the Atomic Force Microscope tip can be used to remove regions of the self-assembling monolayer in order to produce well-defined patterns. If this process is then followed by submersion of the sample into a solution containing neuro-compatible proteins, they will self assemble on these exposed regions of gold, creating well-specified regions for promoted neuron growth.

Keywords

scanning probe microscopy (SPM)biomaterialbiomedical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium Q – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 207 - 212
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Stetner, J., in Self-assembled monolater formation of alkanethiols on gold: Growth in solution versus physical vapor deposition, Doctoral Thesis (Graz University of Technology, 2010), pp. 25–47.Google Scholar
O’Dwyer, C., Gay, G., Viaria de Lesegno, B., and Weiner, J., Langmuir 20, 8172–8182 (2004).CrossRefGoogle Scholar
Ulman, A., Eilers, J.E., and Tillman, N., Langmuir 5, 1147–1152 (1989).CrossRefGoogle Scholar
Zhuang, X., Bartley, L.E., Babcock, H.P., Russel, R., Ha, T., Herschlag, D., and Chu, S., Science 288, 2048–2051 (2000).CrossRefGoogle Scholar
Xu, Song and Liu, Gang-yu, Langmuir 13, 127–129 (1997).CrossRefGoogle Scholar
Franze, K. and Guck, J, Rep. Prog. Phys. 73, 094601 (2010).CrossRefGoogle Scholar
Staii, C., Viesselmann, C., Ballweg, J., Shi, L., Liu, G-y., Williams, J.C., Dent, E.W., Coppersmith, S., and Eriksson, M.A., Biomaterials 30, 3397–3404 (2009).CrossRefGoogle Scholar
Staii, C., Viesselmann, C., Ballweg, J., Williams, J.C., Dent, E.W., Coppersmith, S.N., and Eriksson, M.A., Langmuir, 27, 233–239 (2011).CrossRefGoogle Scholar
Spedden, E., White, J.D., Naumova, E.N., Kaplan, D.L., and Staii, C., Biophys. J. 103, 868–877 (2012).CrossRefGoogle Scholar
Beighley, R., Spedden, E., Sekeroglu, K., Atherton, T., Demirel, M.C., and Staii, C., App. Phys. Lett. 101, 143701 (2012).CrossRefGoogle Scholar