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Bioactive Glass Templates for the Synthesis of Bone-Like Tissue In Vitro

Published online by Cambridge University Press:  15 February 2011

A. Ei-Ghannam ,
P. Ducheyne  and
I. M. Shapiro
A. Ei-Ghannam
Affiliation:
Department of Bioengineering, School of Engineering and Applied Science University of Pennsylvania Philadelphia, PA 19104 U.S.A.
P. Ducheyne
Affiliation:
Department of Bioengineering, School of Engineering and Applied Science University of Pennsylvania Philadelphia, PA 19104 U.S.A.
I. M. Shapiro
Affiliation:
Department of Biochemistry, School of Dental Medicine University of Pennsylvania Philadelphia, PA 19104 U.S.A.
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Abstract

Porous glass templates of pore size 75–200 14m and 20–30% porosity were synthesized. The glass disks were conditioned in a modified tris buffer (SBF) for 48 hrs and then treated with tissue culture medium (TCM) for 1 hr at 37°C. Other porous glass templates were treated with either SBF or TCM. The conditioned glass disks were seeded with 106 neonatal rat calvaria osteoblasts and maintained in culture for 2, 5 or 7 days. It was found that the bioactive glass was rapidly invaded by cells which colonized the porous template. Morphological and biochemical analyses suggested that a bone-like tissue was formed inside templates conditioned with both SBF and TCM. By 7 days, the cells exhibited high alkaline phosphatase activity and synthesized osteocalcin. SDS-PAGE indicated the presence of type I collagen. SEM-EDAX analysis showed the formation of a bone-like tissue throughout the entire sample, while inspection of the Ca:P ratio indicated that the cells synthesized a mineral phase. Indeed, the FTIR spectra of the mineral confirmed that it was a biological hydroxyapatite. The rapid formation of the bone-like material within the template in vitro suggests that this template may be valuable clinically for the repair of bone lesions.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 257
Copyright
Copyright © Materials Research Society 1994

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References

1. Hench, L. L., Splinter, R. J., Allen, W. C., and Greenlee, T. K., J. Biomed. Mater. Res. Symp. 2, 117 (1971).Google Scholar
2. Jarcho, M., Thomas, M. B., Bobick, J., Kay, J. F., and Doremus, B. H., J. Mat. Sci. 11, 2027 (1976).Google Scholar
3. Ducheyne, P., Hench, L. L., Kagan, A., Martens, M., Bursens, A., and Mulier, J. C., J. Biomed. Mater. Res. 14, 225 (1980).Google Scholar
4. Kokubo, T., Ito, S., Huang, Z. T., Hayashi, T., Kitsugi, T., and Yamamuro, T., J. Biomed. Mater. Res. 24, 331 (1990).Google Scholar
5. Brighton, C. T., Schaffer, J. L., Shapiro, D. B., S.Tang, J., and Clark, C. C., J. Orthop. Res. 9, 847 (1991).Google Scholar
6. El-Ghannam, A., Ducheyne, P. and Shapiro, I., 6th International Symposium on ceramics in medicine conference proceeding, Ducheyne, P. and Christianson, D. (eds.), Butterworth & Heinemann Ltd., Oxford, U.K. pp 143, (1993).Google Scholar
7. El-Ghannam, A., Ducheyne, P. and Shapiro, I., J. Biomed. Mater. Res., submitted (1993).Google Scholar
8. Lowry, O. H., Roberts, N. R., Wu, M., Hixon, W. S., and Crawford, E. J., J. Biol. Chem. 207, 19 (1954).Google Scholar
9. Clark, C. C., and Richards, C. F., Coll. Relat. Res. 5, 205 (1985).Google Scholar
10. Gross, U. M., and Strunz, V., J. Biomed. Mater. Res. 19, 251 (1985).Google Scholar