Hostname: page-component-848d4c4894-tn8tq Total loading time: 0 Render date: 2024-06-23T19:14:10.111Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of forcespun fiber-aligned scaffolds from gelatin–zein composites for potential use in tissue engineering and drug release

Published online by Cambridge University Press:  10 May 2018

Narsimha Mamidi Open the ORCID record for Narsimha Mamidi [Opens in a new window] ,
Irasema Lopez Romo ,
Héctor Manuel Leija Gutiérrez ,
Enrique V. Barrera  and
Alex Elías-Zúñiga
Narsimha Mamidi*
Affiliation:
Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México
Irasema Lopez Romo
Affiliation:
Tecnológico de Monterrey, Centro de Biotecnología-FEMSA, School of Engineering and Science, Av. Eugenio Garza Sada 2501, Monterrey, N.L., C.P. 64849, México
Héctor Manuel Leija Gutiérrez
Affiliation:
Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México
Enrique V. Barrera
Affiliation:
Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México Department of Materials Science and NanoEngineering, Rice University, Houston, TX 77005, USA Department of Chemistry, Rice University, Houston, TX 77005, USA
Alex Elías-Zúñiga
Affiliation:
Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México
*
Address all correspondence to Narsimha Mamidi at narsimhachem06@gmail.com
Get access

Abstract

In this study, based on the collection process, three-dimensional aligned fiber scaffolds from gelatin and zein protein were manufactured using Forcespinning®. The homogeneous blending of gelatin:zein (1:4) showed improved tensile and good hydrophobic properties (water contact angle of 115 °C). Cell viability, adhesion, proliferation, and drug release were measured. The cell viability was studied with human fibroblasts and a low cytotoxic effect was observed. Berberine drug release was measured and sustained release rate was observed over 15 days. The morphologic features, prolonged drug release, and cytotoxicity results suggest that these fibers could be appropriate for drug delivery and tissue engineering applications.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 3 , September 2018 , pp. 885 - 892
Check for updates
Copyright
Copyright © Materials Research Society 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Flemming, R.G., Murphy, C.J., Abrams, G.A., Goodman, S.L., and Nealey, P.F.: Effects of synthetic micro- and nano-structured surfaces on cell behavior. Biomaterials 20, 573 (1999).Google Scholar
2.Tay, C.Y., Irvine, S.A., Boey, F.Y.C., Tan, L.P., and Venkatraman, S.: Micro-/nano-engineered cellular responses for soft tissue engineering and biomedical applications. Small 7, 1361 (2011).Google Scholar
3.Kim, H.N., Jiao, A., Hwang, N.S., Kim, M.S., Kang, D.H., Kim, D.H., and Suh, K.Y.: Nanotopography-guided tissue engineering and regenerative medicine. Adv. Drug. Deliv. Rev. 65, 536 (2013).Google Scholar
4.Xin, X., Hussain, M., and Mao, J.J.: Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffold. Biomaterials 28, 316 (2007).Google Scholar
5.Yoshimoto, H., Shin, Y.M., Terai, H., and Vacanti, J.P.: A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials 24, 2077 (2003).Google Scholar
6.Wade, R.J., Bassin, E.J., Rodell, C.B., and Burdick, J.A.: Protease-degradable electrospun fibrous hydrogels. Nat. Commun. 6, 6639 (2015).Google Scholar
7.Tan, Y.J., Tan, X., Yeong, W.Y., and Tor, S.B.: Additive manufacturing of patient-customizable scaffolds for tubular tissues using the melt-drawing method. Materials 9, 893 (2016).Google Scholar
8.Wang, L., Chang, M.W., Ahmad, Z., Zheng, H., and Li, J.S.: Mass and controlled fabrication of aligned PVP fibers for matrix type antibiotic drug delivery systems. Chem. Eng. J. 307, 661 (2017).Google Scholar
9.Kazantseva, J., Ivanov, R., Gasik, M., Neuman, T., and Hussainova, I.: Graphene-augmented nanofiber scaffolds demonstrate new features in cells behaviour. Sci. Rep. 6, 30150 (2016).Google Scholar
10.Fereshteh, Z., Fathi, M., Bagri, A., and Boccaccini, A.R.: Preparation and characterization of aligned porous PCL/zein scaffolds as drug delivery systems via improved unidirectional freeze-drying method. Mater. Sci. Eng. 68, 613 (2016).Google Scholar
11.Moon, Y.W., Choi, I.J., Koh, Y.H., and Kim, H.E.: Porous alumina ceramic scaffolds with biomimetic macro/micro-porous structure using three-dimensional (3-D) ceramic/camphene-based extrusion. Ceram. Int. 41, 12371 (2015).Google Scholar
12.Bai, H., Walsh, F., Gludovatz, B., Delattre, B., Huang, C., Chen, Y., Tomsia, A.P., and Ritchie, R.O.: Bioinspired hydroxyapatite/poly(methyl methacrylate) composite with a nacre-mimetic architecture by a bidirectional freezing method. Adv. Mater. 28, 50 (2016).Google Scholar
13.Das, S., Sharma, M., Saharia, D., Sarma, K.K., Sarma, M.G., Borthakur, B.B., and Bora, U.: Data in support of in vivo studies of silk based gold nano-composite conduits for functional peripheral nerve regeneration. Data Breif 4, 315 (2015).Google Scholar
14.Wise, J.K., Yarin, A.L., Megaridis, C.M., and Cho, M.: Chondrogenic differentiation of human mesenchymal stem cells on oriented nanofibrous scaffolds: engineering the superficial zone of articular cartilage. Tissue Eng. Part A 15, 913 (2009).Google Scholar
15.Chaurey, V., Block, F., Su, Y.H., Chiang, P.C., Botchwey, E., Chou, C.F., and Swami, N.S.: Nanofiber size-dependent sensitivity of fibroblast directionality to the methodology for scaffold alignment. Acta Biomater. 8, 3982 (2012).Google Scholar
16.Kim, J.I., Hwang, T.I., Aguilar, L.E., Park, C.H., and Kim, C.S.A.: Controlled design of aligned and random nanofibers for 3D bi-functionalized nerve conduits fabricated via a novel electrospinning set-up. Sci. Rep. 6, 23761 (2016).Google Scholar
17.Wang, C.Y., Zhang, K.H., Fan, C.Y., Mo, X.M., Ruan, H.J., and Li, F.F.: Aligned natural-synthetic polyblend nanofibers for peripheral nerve regeneration. Acta Biomater. 7, 634 (2011).Google Scholar
18.Park, S.H., Kim, M.S., Lee, B., Park, J.H., Lee, H.J., Lee, N.K., Jeon, N.L., and Suh, K.Y.: Creation of a hybrid scaffold with dual configuration of aligned and random electrospun fibers. ACS Appl. Mater. Interfaces 8, 2826 (2016).Google Scholar
19.Feng, J., Zhang, D., Zhu, M., and Gao, C.: Poly(L-lactide) melt spun fiber-aligned scaffolds coated with collagen or chitosan for guiding the directional migration of osteoblasts in vitro. J. Mater. Chem. B 5, 5176 (2017).Google Scholar
20.Fernandez, A., Torres-Giner, S., and Lagaron, J.M.: Novel route to stabilization of bioactive antioxidants by encapsulation in electrospun fibers of zein prolamine. Food Hydrocoll. 23, 1427 (2009).Google Scholar
21.Mamidi, N., Leija, H.M., Villela, J., Isenhart, L., Barrera, E.V., and Elías, A.: Fabrication of gelatin-poly(epichlorohydrin-co-ethylene oxide) fiber scaffolds by Forcespinning® for tissue engineering and drug release. MRS Commun. 7, 913 (2017).Google Scholar
22.Gönen, S.Ö., Erol, M., and Küçükbayrak, S.: Evaluation of the factors influencing the resultant diameter of the electrospun gelatin/sodium alginate nanofibers via Box-Behnken design. Mater. Sci. Eng. C 58, 709 (2016).Google Scholar
23.Corradini, E., Curti, P.S., Meniqueti, A.B., Martins, A.F., Rubira, A.F., and Muniz, E.C.: Recent advances in food-packing, pharmaceutical and biomedical applications of zein and zein-based materials. Int. J. Mol. Sci. 15, 22438 (2014).Google Scholar
24.Paliwal, R. and Palakurthi, S.: Zein in controlled drug delivery and tissue engineering. J. Control. Release 189, 108 (2014).Google Scholar
25.Sarkar, K., Gomez, C., Zambrano, S., Ramirez, M., De Hoyos, E., Vasquez, H., and Lozano, K.: Electrospinning to Forcespinning™. Mater. Today 13, 12 (2010).Google Scholar
26.Guo, C., Zhou, L., and Lv, J.: Effects of expandable graphite and modified ammonium polyphosphate on the flame-retardant and mechanical properties of wood flour-polypropylene composites. Polym. Polym. Compos. 21, 449 (2013).Google Scholar
27.Kong, W., Zhao, Y., Xiao, X., Jin, C., Liu, Y., and Li, Z.: Comparison of anti-bacterial activity of four kinds of alkaloids in Rhizoma coptidis based on microcalorimetry. Chin. J. Chem. 27, 1186 (2009).Google Scholar
28.Yu, H.H., Kim, K.J., Cha, J.D., Kim, H.K., Lee, Y.E., Choi, N.Y., and You, Y.O.: Antimicrobial activity of berberine alone and in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. J. Med. Food 8, 254 (2005).Google Scholar