Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-23T12:14:46.477Z Has data issue: false hasContentIssue false
  • English
  • Français

Optically and Chemically Encoded Nanoparticle Materials for DNA and Protein Detection

Published online by Cambridge University Press:  31 January 2011

C. Shad Thaxton ,
Nathaniel L. Rosi  and
Chad A. Mirkin
Get access

Abstract

Nanoscale materials are beginning to have an impact in the field of molecular diagnostics. In particular, gold nanoparticles surface-functionalized with DNA have garnered much recent interest. Due to the unusual optical and catalytic properties of gold nanoparticle labels, several distinct advantages for assay readout have been realized. This review focuses on the progress made in our group over the past seven years in the development of particle surface chemistry and ultrasensitive protein and nucleic acid assays based upon DNA-functionalized gold nanoparticles. For DNA targets, experiments demonstrate that assays based upon gold nanoparticle labels have enhanced target specificity and in certain cases the sensitivity of polymerase chain reaction (PCR), without the need for target amplification. For protein targets, similar experiments demonstrate that assays based upon gold nanoparticles are up to one million times more sensitive than conventional protein detection methods. Recent data using human samples demonstrate the utility of such assays.

Keywords

bio-barcode assayDNA detectiongold nanoparticlesmetallic nanostructuresprotein detection.
Type
Research Article
Information
MRS Bulletin , Volume 30 , Issue 5: Synthesis and Plasmonic Properties of Nanostructures , May 2005 , pp. 376 - 380
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Mirkin, C.A., Letsinger, R.L., Mucic, R.C., and Storhoff, J.J., Nature 382 (1996) p. 607.Google Scholar
2.Storhoff, J.J., Elghanian, R., Mucic, R.C., Mirkin, C.A., and Letsinger, R.L., J. Am. Chem. Soc. 120 (1998) p. 1959.CrossRefGoogle Scholar
3.Elghanian, R., Storhoff, J.J., Mucic, R.C., Letsinger, R.L., and Mirkin, C.A., Science 277 (1997) p. 1078.CrossRefGoogle Scholar
4.Jin, R.C., Wu, G.S., Li, Z., Mirkin, C.A., and Schatz, G.C., J. Am. Chem. Soc. 125 (2003) p. 1643.CrossRefGoogle Scholar
5.Cao, Y.W., Jin, R., and Mirkin, C.A., J. Am. Chem. Soc. 123 (2001) p. 7961.Google Scholar
6.Reynolds, R.A., Mirkin, C.A., and Letsinger, R.L., J. Am. Chem. Soc. 122 (2000) p. 3795.Google Scholar
7.Storhoff, J.J., Marla, S.S., Garimella, V., and Mirkin, C.A., in Microarray Technology and its Applications, edited by Müller, U.R. and Nicolau, D.V. (Springer, Berlin, 2005) p. 147.Google Scholar
8.Yguerabide, J. and Yguerabide, E.E., J. Cell. Biochem. Suppl. 37 (2001) p. 71.Google Scholar
9.Taton, T.A., Lu, G., and Mirkin, C.A., J. Am. Chem. Soc. 123 (2001) p. 5164.Google Scholar
10.Taton, T.A., Mirkin, C.A., and Letsinger, R.L., Science 289 (2000) p. 1757.CrossRefGoogle Scholar
11.Storhoff, J.J., Lucas, A.D., Garimella, V., Bao, Y.P., and Muller, U.R., Nature Biotech. 22 (2004) p. 883.CrossRefGoogle Scholar
12.Storhoff, J.J., Marla, S.S., Bao, P., Hagenow, S., Mehta, H., Lucas, A., Garimella, V., Patno, T., Buckingham, W., Cork, W., and Müller, U.R., Biosen. & Bioelec. 19 (2004) p. 875.CrossRefGoogle Scholar
13.Cao, Y.W.C., Jin, R.C., and Mirkin, C.A., Science 297 (2002) p. 1536.CrossRefGoogle Scholar
14.Cao, Y.C., Jin, R.C., Nam, J.M., Thaxton, C.S., and Mirkin, C.A., J. Am. Chem. Soc. 125 (2003) p. 14676.CrossRefGoogle Scholar
15.MacBeath, G. and Schreiber, S.L., Science 289 (2000) p. 1760.CrossRefGoogle Scholar
16.Nam, J.M., Thaxton, C.S., and Mirkin, C.A., Science 301 (2003) p. 1886.CrossRefGoogle Scholar
17.Catalona, W.J., Prostate (1996) p. 64.3.0.CO;2-J>CrossRefGoogle ScholarPubMed
18.Ferguson, R.A., Yu, H., Kalyvas, M., Zammit, S., and Diamandis, E., Clin. Chem. 42 (1996) p. 675.Google Scholar
19.Chang, L., Bakhos, L., Wang, Z.Q., Venton, D.L., and Klein, W.L., J. Mol. Neurosci. 20 (2003) p. 305.CrossRefGoogle Scholar
20.Georganopoulou, D.G., Chang, L., Nam, J.-M., Thaxton, C.S., Mufson, E.J., Klein, W.L., and Mirkin, C.A., Proc. Natl. Acad. Sci. U.S.A. 102 (2005) p. 2273.Google Scholar
21.Nam, J.M., Stoeva, S.I., and Mirkin, C.A., J. Am. Chem. Soc. 126 (2004) p. 5932.CrossRefGoogle Scholar
22.Rosi, N.L. and Mirkin, C.A., Chem. Rev. (2005) in press.Google Scholar