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Atomic Force Microscopy of Biological Samples

Published online by Cambridge University Press:  31 January 2011

P.L.T.M. Frederix ,
B.W. Hoogenboom ,
D. Fotiadis ,
D.J. Müller  and
A. Engel
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Abstract

The atomic force microscope (AFM) allows biomolecules to be observed and manipulated under native conditions. It produces images with an outstanding signal-to-noise ratio and addresses single molecules while the sample is in a buffer solution. Progress in sample preparation and instrumentation has led to topographs that reveal subnanometer details and the surface dynamics of biomolecules. Tethering single molecules between a support and a retracting AFM tip produces force–extension curves, giving information about the mechanical stability of secondary structural elements. For both imaging and force spectroscopy, the cantilever and its tip are critical:the mechanical properties of the cantilever dictate the force sensitivity and the scanning speed, whereas the tip shape determines the achievable lateral resolution.

Keywords

atomic force microscopybiological moleculesimagingnative membranesprotein dynamicssingle-molecule force spectroscopy.
Type
Research Article
Information
MRS Bulletin , Volume 29 , Issue 7: Scanning Probe Microscopy in Materials Science , July 2004 , pp. 449 - 455
Copyright
Copyright © Materials Research Society 2004

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