Hostname: page-component-77c89778f8-fv566 Total loading time: 0 Render date: 2024-07-18T11:35:32.875Z Has data issue: false hasContentIssue false
  • English
  • Français

The use of magnetic nanoparticles for immobilization and recycling of enzymes

Published online by Cambridge University Press:  13 August 2018

Shirley Furtado ,
Mariana Brandes ,
Catalina Alamón ,
Santiago Botasini  and
Ana M.B. Cantera
Shirley Furtado
Affiliation:
Cátedra de Bioquímica /Dpto. de Biociencias/Facultad de Química/Universidad de la República, Montevideo, Uruguay Laboratorio de Enzimas Hidrolíticas/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Mariana Brandes
Affiliation:
Laboratorio de Biomateriales/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Catalina Alamón
Affiliation:
Laboratorio de Biomateriales/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Santiago Botasini*
Affiliation:
Laboratorio de Biomateriales/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Ana M.B. Cantera
Affiliation:
Cátedra de Bioquímica /Dpto. de Biociencias/Facultad de Química/Universidad de la República, Montevideo, Uruguay Laboratorio de Enzimas Hidrolíticas/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
*
*(Email: sbotasini@fcien.edu.uy)
Get access

Abstract

The use of nanostructured materials for enzyme immobilization is an active field of research due to its large surface area and the new emergent properties derived from its size. The present work is focused on the synthesis of magnetic nanoparticles for the adsorption of cysteine-proteolytic enzymes extracted from Bromelia antiacantha Bertol (Bromeliaceae) fruit. The results show that enzyme adsorption is highly dependent on the temperature and pH. The biocatalyst activity increased up to 40 %, once immobilized onto the magnetic nanoparticles. In addition, they can be recovered using a magnet allowing them to be reused up to 5 cycles with a marginal loss (5 %) of the initial activity.

Keywords

ferromagneticmagneticnanostructurecatalytic
Type
Articles
Information
MRS Advances , Volume 3 , Issue 61: Materials Science Young Researchers of Uruguay , 2018 , pp. 3581 - 3587
Copyright
Copyright © Materials Research Society 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bornscheuer, U. T., Angew. Chemie - Int. Ed. 42, 3336 (2003).CrossRefGoogle Scholar
Quiróga, A. I. E., Obregón, W.D., CYTED 221 (2009).Google Scholar
Hosseinipour, S. L., Khiabani, M. S., Hamishehkar, H., and Salehi, R., J. Nanoparticle Res. 17, 382 (2015).CrossRefGoogle Scholar
Furtado, S. and Cantera, A.M. B., Prep. Biochem. Biotechnol. 32, 217 (2002).CrossRefGoogle Scholar
Min, K. and Yoo, Y. J., Biotechnol. Bioprocess Eng. 19, 553 (2014).CrossRefGoogle Scholar
Mosafa, L., Moghadam, M., and Shahedi, M., Chinese J. Catal. 34, 1897 (2013).CrossRefGoogle Scholar
Dyal, A., Loos, K., Noto, M., Chang, S. W., Spagnoli, C., Shafi, K. V. P. M., Ulman, A., Cowman, M., and Gross, R. a, J. Am. Chem. Soc. 125, 1684 (2003).CrossRefGoogle Scholar
Wang, W., Xu, Y., Wang, D. I. C., and Li, Z., J. Am. Chem. Soc. 131, 12892 (2009).CrossRefGoogle Scholar
Jiang, Y., Guo, C., Xia, H., Mahmood, I., Liu, C., and Liu, H., J. Mol. Catal. B Enzym. 58, 103 (2009).CrossRefGoogle Scholar
Ansari, S. A. and Husain, Q., Biotechnol. Adv. 30, 512 (2012).CrossRefGoogle Scholar
Vaghari, H., Jafarizadeh-Malmiri, H., Mohammadlou, M., Berenjian, A., Anarjan, N., Jafari, N., and Nasiri, S., Biotechnol. Lett. 38, 223 (2016).CrossRefGoogle Scholar
Vallés, D., Furtado, S., and Cantera, A.M. B., Enzyme Microb. Technol. 40, 409 (2007).CrossRefGoogle Scholar
Tancredi, P., Botasini, S., Moscoso-Lodoño, O., Méndez, E., and Socolovsky, L., Colloids Surfaces A Physycichen. Eng. Asp. 464, 46 (2015).CrossRefGoogle Scholar
Bradford, M. M., 254, 248 (1976).Google Scholar
Castro, S. and Cantera, A. M. B., Biotecnol. Apl. 13, 213 (1996).Google Scholar
Andrews, B. A. and Asenjo, J. A., Biotechnol. Bioeng. 28, 1366 (1986).CrossRefGoogle Scholar
Vallés, D., Bruno, M., López, L. M. I., Caffini, N. O., and Cantera, A. M. B., Protein J . 27, 267 (2008).CrossRefGoogle Scholar
Bayramoǧlu, G., Yilmaz, M., and Arica, M. Y., Food Chem . 84, 591 (2004).CrossRefGoogle Scholar
Reshmi, R., Sanjay, G., and Sugunan, S., Catal. Commun. 7, 460 (2006).CrossRefGoogle Scholar