Hostname: page-component-848d4c4894-8kt4b Total loading time: 0 Render date: 2024-06-24T11:28:04.825Z Has data issue: false hasContentIssue false
  • English
  • Français

Preparation and Characterization of Chitosan Microspheres for Controlled Release of Rh-BMP-2

Published online by Cambridge University Press:  18 November 2019

Cindy Sinaí Velázquez-González ,
Ena Athenea Aguilar-Reyes ,
Rosa Elvira Núñez-Anita  and
Carlos Alberto León-Patiño Open the ORCID record for Carlos Alberto León-Patiño [Opens in a new window]
Cindy Sinaí Velázquez-González
Affiliation:
Instituto de Investigación en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México.
Ena Athenea Aguilar-Reyes*
Affiliation:
Instituto de Investigación en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México.
Rosa Elvira Núñez-Anita
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Unidad de Proteómica y Bioingeniería Celular, Universidad Michoacana de San Nicolás de Hidalgo, Tarímbaro, México.
Carlos Alberto León-Patiño
Affiliation:
Instituto de Investigación en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México.
*
*Corresponding author: Dr. Ena Athenea Aguilar Reyes (Email: aareyes@umich.mx)
Get access

Abstract

The implantation and controlled release of growth factors can enhance the proliferation and differentiation of cells that promote new bone formation at defect sites. Therefore, chitosan polymer microspheres were prepared by the water-in-oil emulsion (W/O) method and solvent freeze-drying, using glutaraldehyde as an ionic crosslinker, along with the lyophilization of solvents, to microencapsulate growth factors, preventing denaturation. The microspheres were loaded with recombinant bone morphogenetic protein 2 (Rh-BMP-2). They were spherical in shape, with a rough surface ranging in particle size from 0.4 to 1.6 μm. The yield percentage with respect to the polymer was 70% and the BMP-2 load was regulated by the initial protein dose. BMP-2 release experiments were performed for 7 days in PBS solutions at pH 4 and 7.4. The results showed that the protein release rate was only 2% lower at pH 7.4. BMP-2/chitosan microspheres were compatible with the MG-63 cell line (ATCC®CRL-1427™Homo sapiens bone osteosarcoma) and could be considered drug delivery vehicles in bone tissue engineering applications.

Keywords

biomaterialbonefreeze dryingpolymer
Type
Articles
Information
MRS Advances , Volume 4 , Issue 59-60: International Materials Research Congress XXVIII , 2019 , pp. 3259 - 3267
Copyright
Copyright © Materials Research Society 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Villicaña-Molina, E., Pacheco-Contreras, E., Aguilar-Reyes, E. A., León-Patiño, C. A., Int. J. Polym. Mater. Polym. Biomat. in press (2019).Google Scholar
Zhu, S., Lu, P., Liu, H., Chen, P., Wu, Y., Wang, Y., Sun, H., Zhang, X., Ann. Rheum Dis. 74, 285-293 (2015).CrossRefGoogle Scholar
Zeng, W., Rong, M., Hu, X., Xiao, W., Qi, F., Huang, J., Luo, Z., PLOS ONE 9 (7), 1-9 (2014).Google Scholar
Onwulata, C., J. Food Process Preserv. 37, 1-23 (2012).Google Scholar
Lupo, B., Gonzalez, C., Maestro, A., Rev. Venez. Cienc. Tecnol. Aliment. 3 (1), 130-151 (2012).Google Scholar
Agnihotri, S. A., Mallinkarjuna, N. N., Aminabhavi, T. M., J. Control. Release 100 (1), 5-28 (2004).CrossRefGoogle Scholar
Ahsan, S. M., Thomas, M., Reddy, K. K., Sooraparaju, S. G., Asthana, A., Bhatnagar, I., Int. J. Biol. Macromol. 110, 97-109 (2018).CrossRefGoogle Scholar
Lee, S. S., Huang, B. J., Kaltz, S. R., Sur, S., Newcomb, C. J., Stock, S. R., Shah, R. N., Stupp, S. I., Biomaterials 34 (2), 452-459 (2013).CrossRefGoogle Scholar
Bigham-Sadegh, A., Mirshokraei, P., Karimi, I., Oryan, A., Aparviz, A., Shafiei-Sarvestani, Z., Connect. Tissue Res. 53 (4), 334-342 (2012).CrossRefGoogle Scholar
Cho, T.-J., Gerstenfeld, L., Einhorn, T. A., J. Bone Miner. Res. 17, 5113-520, (2002).CrossRefGoogle Scholar
Oryan, A., Bigham-Sadegh, A., Abbasi-Teshnizi, F., Bone 63, 53-60 (2014).CrossRefGoogle ScholarPubMed
Urist, M. R., J. Bone Miner. Res. 12, 343-346 (1997).CrossRefGoogle Scholar
Jain, A., Pundir, S., Sharma, A., J. Indian Soc. Periodontol. 17, 583-586 (2013).CrossRefGoogle Scholar
Carreira, A. C., Lojudice, F. H., Halcsik, E., Navarro, R. D., Sogayar, M.C., Granjeiro, J. M., J. Dent. Res. 10, 1-11, (2014).Google Scholar
Cottrell, J. A., Keane, O., Lin, S. S., O’Connor, J. P., J. Appl. Biomed. 12, 127-135 (2014).CrossRefGoogle Scholar
Olivera, A. D., Barreiro, M. F., Lopretti, M., Rev. Iberoam. Polim. 13 (5), 238-244 (2012).Google Scholar
Sandoval, P. A., Baena, Y., Aragón, M., Rosas, J. E., Ponce D’Leon, L. F., Rev. Colomb. Cienc. Quim. Farm. 37 (2), 105-121(2008).Google Scholar
Zou, X., Zhao, X., Ye, L., Wang, Q., Li, H., Ind. Eng. Chem. Res. 21, 1389-1397(2015).CrossRefGoogle Scholar
O’Hagan, D. T., Singh, M., Ulmer, J. B., Methods 40 (1), 10-19 (2006).CrossRefGoogle Scholar
Wei, W., Yuan, L., Hu, G., Wang, L.-Y., Wu, J., Hu, X., Su, Z.-G., Ma, G.-H., Adv. Mater. 20, 2292-2296 (2008).CrossRefGoogle Scholar