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The Aqueous Two Phase System (ATPS) Deserves Plausible Real-World Modeling for the Structure and Function of Living Cells

Published online by Cambridge University Press:  15 May 2017

Kanta Tsumoto  and
Kenichi Yoshikawa
Kanta Tsumoto*
Affiliation:
Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan
Kenichi Yoshikawa
Affiliation:
Faculty of Life and Medical Sciences, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan
*
*(Email: tsumoto@chem.mie-u.ac.jp)
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Abstract

An aqueous two phase system (ATPS) is composed of binary hydrophilic polymers, for example, polyethylene glycol (PEG) and dextran, under an immiscible condition, and can also exhibit micro-segregation to produce cell-sized microcompartments like water-in-water microdroplets. Without membranes, interestingly, the microdroplet can serve as a micro-vessel (reactor) that contains various biochemical macromolecules like DNAs and proteins. We here present that PEG/dextran ATPS micro-segregation can provide an effective soft boundary to separate these biochemical macromolecules from the external environment. Trapped DNAs and proteins were concentrated inside such small spaces, and therefore, their interaction could be highly promoted to cause passive aggregation and controlled cross-linking if a certain cross-linker was added. We believe that the ATPS microdroplets might be associated with complicated structures and functions of living cells.

Keywords

biomaterialbiomimetic (assembly)polymer
Type
Articles
Information
MRS Advances , Volume 2 , Issue 45: Soft Materials and Biomaterials , 2017 , pp. 2407 - 2413
Copyright
Copyright © Materials Research Society 2017 

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References

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