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Towards a single bioactive substrate combining SERS-effect and drug release control based on thin anodic porous alumina coated with gold and with lipid bilayers

Published online by Cambridge University Press:  05 January 2017

Amirreza Shayganpour*
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy Department of Bioengineering and Robotics, University of Genova, viale Causa 13, I-16145 Genova, Italy
Marco Salerno
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy
Barbara Salis
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy Department of Bioengineering and Robotics, University of Genova, viale Causa 13, I-16145 Genova, Italy
Silvia Dante
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy
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Thin anodic porous alumina (tAPA), engineered by electrochemical anodization of aluminum and post-fabrication etching, has already shown surface-enhanced Raman scattering (SERS) activity, after overcoating with a thin gold film. On the other hand, the tAPA nanoporous surface, which is biocompatible and presents controlled roughness, has been extensively investigated as a substrate for living cell cultures. Here, we are interested in exploiting the nanoporosity of tAPA as a drug reservoir and demonstrating drug-delivery capabilities of these substrates which can be combined with the above cell-seeding and SERS activities. We focused on the loading/elution of a test drug, the nonsteroidal anti-inflammatory and analgesic molecule Diclofenac. We carried out pore loading of differently concentrated aqueous solutions of the test drug, and characterized the elution profiles by UV-Vis absorbance, using the lipid bilayers coated on the top surface as a mechanism for retarded elution from the pores, providing a more sustained release. We also demonstrated that, by changing an environmental parameter such as the pH, we can trigger an increased release of the drug. Additionally, we investigated the tAPA adsorption properties by quartz crystal microbalance technique with dissipation monitoring (QCM-D). For the purpose, anodization was carried out on an Al-coated quartz, which resulted in successful fabrication of tAPA on the sensor. Finally, the process of lipid bilayer formation on the nanoporous sensor, as well as the test drug loading, was demonstrated by QCM-D.

Copyright © Materials Research Society 2017 

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these two authors have equally contributed to the work



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