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Three-Partition Hydrogel Apparatus to Gauge Viability of Gelatin-Pluronic® F127 Hybrid Hydrogels as Cell Barrier Membranes for Guided Bone Regeneration Following Periodontitis

Published online by Cambridge University Press:  06 February 2019

Aaron Z. Sun ,
Kevin H. Chen ,
Joon Young Lee ,
Juyi Li  and
Miriam Rafailovich
Aaron Z. Sun
Affiliation:
Ed W Clark High School, Las Vegas, NV89102, U.S.A.
Kevin H. Chen*
Affiliation:
Mira Costa High School, Manhattan Beach, CA90266, U.S.A.
Joon Young Lee
Affiliation:
Seoul International School, Seongnam City, South Korea13113
Juyi Li
Affiliation:
Dept. Mat. Sci. and Eng., Stony Brook University, Stony Brook, NY11790, U.S.A.
Miriam Rafailovich
Affiliation:
Dept. Mat. Sci. and Eng., Stony Brook University, Stony Brook, NY11790, U.S.A.
*
*(Email: kevinchenyy@gmail.com)
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Abstract

Periodontitis, or conventionally “Gum Disease,” is the infection and inflammation of gingival tissue, and is currently the leading cause of tooth loss in the United States. One of the most effective treatments of periodontitis is guided bone regeneration (GBR); however, current GBR barrier membranes lack high biocompatibility and cell impermeability. The authors of this study evaluated the in vitro viability of previously synthesized Gelatin-Pluronic® F127 hybrid hydrogels as potential GBR barrier membranes through a novel three-partition test involving migration of fluorescent-dyed human dermal fibroblasts. Results showed that cells were unable to migrate across the Gelatin-Pluronic® F127 hybrid hydrogel barrier, whereas control setups with gelatin hydrogel barriers showed cell permeability. In addition, cytotoxicity tests were conducted with fibroblasts plated in both cell mediums that had been incubated while in contact hybrid gels and cell mediums suspended on the surface of hybrid gels during swelling procedures. Fluorescence cell plate readings showed similar cell viability across data from both tests, indicating that Gelatin-Pluronic® F127 hybrid hydrogels are not toxic to cells, and thus biocompatible.

Keywords

biomaterialbiologicalcellular (material type)
Type
Articles
Information
MRS Advances , Volume 4 , Issue 21: Biomaterials and Soft Materials , 2019 , pp. 1223 - 1229
Copyright
Copyright © Materials Research Society 2019 

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Footnotes

The Principal Authors Aaron Z. Sun, Kevin H. Chen, and Joon Young Lee contributed equally to the research project on which this article is based.

References

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