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Modulating the lifetime of the charge-separated state in photosynthetic reaction center by out-of-protein electrostatics

Published online by Cambridge University Press:  01 March 2018

Francesco Milano ,
Roberto R. Tangorra ,
Angela Agostiano ,
Livia Giotta ,
Vincenzo De Leo ,
Fulvio Ciriaco  and
Massimo Trotta
Francesco Milano
Affiliation:
CNR-IPCF, Istituto per i Processi Chimico Fisici – Consiglio Nazionale delle Ricerche – Via Orabona, 4 – 70125 BARI.
Roberto R. Tangorra
Affiliation:
Departmento of Chemistry – Università degli Studi di Bari, Via Orabona, 4 – 70125 BARI.
Angela Agostiano
Affiliation:
CNR-IPCF, Istituto per i Processi Chimico Fisici – Consiglio Nazionale delle Ricerche – Via Orabona, 4 – 70125 BARI. Departmento of Chemistry – Università degli Studi di Bari, Via Orabona, 4 – 70125 BARI.
Livia Giotta
Affiliation:
DiSTeBA Department of Biology and Environmental Science and Technology – Università del Salento, Via Provinciale Lecce Monteroni 165, I-73100 Lecce, Italy.
Vincenzo De Leo
Affiliation:
Departmento of Chemistry – Università degli Studi di Bari, Via Orabona, 4 – 70125 BARI.
Fulvio Ciriaco
Affiliation:
Departmento of Chemistry – Università degli Studi di Bari, Via Orabona, 4 – 70125 BARI.
Massimo Trotta*
Affiliation:
CNR-IPCF, Istituto per i Processi Chimico Fisici – Consiglio Nazionale delle Ricerche – Via Orabona, 4 – 70125 BARI.
*
#(Email: massimo.trotta@cnr.it)
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Abstract

The photosynthetic reaction center (RC) is an integral membrane protein that, upon absorption of photons, generates a hole-electron couple with a yield close to one. This energetic state has numerous possible applications in several biotechnological fields given that its lifetime is long enough to allow non-metabolic ancillary redox chemistry to take place. Here we focus on RCs reconstituted in liposomes, formed with sole phospholipids or in blends with other lipids, and show that the electrical charge sitting on the polar head of such hydrophobic molecules does play an important role on the stability of the hole-electron couple. More specifically this study shows that the presence of negative charges in the surrounding of the protein stabilizes the charge-separated state while positive charges have a strong opposite effect.

Keywords

biomimetic (assembly)biomaterialphotochemical
Type
Articles
Information
MRS Advances , Volume 3 , Issue 27: Biomaterials and Soft Materials , 2018 , pp. 1497 - 1507
Copyright
Copyright © Materials Research Society 2018 

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Footnotes

$

Present address: Mossi & Ghisolfi Group, strada Ribrocca 11 - 15057 Tortona, Italy

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