Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-21T12:59:54.119Z Has data issue: false hasContentIssue false
  • English
  • Français

TEM of Paraffin-Embedded H&E- Stained Sections for Viral Diagnosis (An Unusual Papovavirus Case)

Published online by Cambridge University Press:  14 March 2018

Januario C. Estrada ,
M. Angelica Selim  and
Sara E. Miller
Januario C. Estrada
Affiliation:
Department of Pathology, Duke University Medical Center, DurhamNC
M. Angelica Selim
Affiliation:
Department of Pathology, Duke University Medical Center, DurhamNC
Sara E. Miller*
Affiliation:
Department of Pathology, Duke University Medical Center, DurhamNC
*
mille012@mc.duke.edu

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Viral infections can be focal and therefore difficult to find by electron microscopy. In addition to sampling limitations, sometimes the only specimen available for examination is tissue that has already been prepared for light microscopy (LM). We have diagnosed a papovavirus infection in skin by embedding hematoxylin and eosin (H&E)-stained sections for ultrathin sectioning and transmission electron microscopy (TEM).

Immunosuppressed patients can have unusual infections or infections in unusual locations. These agents may be difficult to identify due to their not being suspected and incorrect tests being ordered.

Type
Research Article
Information
Microscopy Today , Volume 13 , Issue 5 , September 2005 , pp. 22 - 25
Copyright
Copyright © Microscopy Society of America 2005

References

[1] Howell, D. N., Miller, S. E.. Meth. Enzymol. 307 (1999) 573.Google Scholar
[2] Miller, S. E.. In Jones, B. R. (ed.) Electron Microscopy, Library Research Associates, Inc., Monroe, NY (1985) p. 293.Google Scholar
[3] van den Bergh Weerman, M. A., Dingemans, K.P.. Ultrastruct. Pathol. 7 (1984) 55.CrossRefGoogle Scholar
[4] Maunsbach, A. B., Afzelius, B. A.. Biomedical Electron Microscopy, Academic Press, New York (1999) p. 28.Google Scholar
[5] Kupke, K. G. et al. J. Electron Microsc. Tech. 1 (1984) 299.Google Scholar
[6] Haycox, C. L. et al. J. Investig. Dermatol. Symp. Proc. 4 (1999) 268.Google Scholar