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Preparation Of Soil Samples For Light And Transmission Electron Microsopy

Published online by Cambridge University Press:  14 March 2018

Gordon Vrdoljak
Gordon Vrdoljak*
Affiliation:
UC Berkeley
*
vrdoljak@uclink.berkeley.edu

Extract

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Soil structure influences water supply to plant roots, aeration, water infiltration rates, suitability of soil medium for seed germination and growth, growth of plant roots, drainage, evaporation, mechanical strength, and workability (Dexter 1988). Adequate description of soil structure for cultivation, engineering, or remediation is typically done by light microscopy and transmission electron microscopy. Literature exists in numerous sources for preparation of soils for microscopy, but often preparation steps are left out due to the shortening of Methods Sections in journal articles to conserve print space. I present here, protocols I've used for preparation of tropical soils (Oxisols) for microscopy.

Type
Research Article
Information
Microscopy Today , Volume 11 , Issue 5 , August 2003 , pp. 38 - 41
Copyright
Copyright © Microscopy Society of America 2003

References

Dexter, A. R., 1988. Advances in characterization of soil structure. Soil and Tillage Res ., 11, 199–238.CrossRefGoogle Scholar
FitzPatrick, E.A., 1993. Soil Microscopy and Micro morphology. John Wiley & Sons, New York.Google Scholar
Spurr, A.R., 1969. A low-viscosity resin embedding medium for electron microscopy. J. Ultra structure Res., 26, 31-43.CrossRefGoogle Scholar
Sudo, T. and Takahashi, H. (1971) The Chlorites and Interstratified Minerals. In: J.A. Gard (ed) The Electron-Optical Investigation of Clays. Mineralogical Society, Monograph 3. London, England, Mineralogical Society, 293.Google Scholar
Vrdoljak, G. and Sposito, G. (2002) Soil Aggregate Hierarchy in a Brazilian Oxisol. In: Violante, P.M. Huang, J.-M. Bollag. L. Gianfreda (eds) Developments in Soil Science, Volume 28 A. Elsevier Science, 197-217.CrossRefGoogle Scholar
Weyda, F., 1990. Notes On The Use Of Nanoplast Fb 101 For Transmission Electron Microscopy. J. of Electron Microscopy Technique , 16, 356–357.Google Scholar