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Out with the Old, In with the New

Published online by Cambridge University Press:  14 March 2018

Stephen W. Carmichael*
Affiliation:
Mayo Clinic

Extract

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Temporal resolution has long been a challenge to microscopists. Certainly, spatial resolution has occupied center stage, but we're all concerned about what happens over time in a biologic system, for example, a cell. Tags such as green fluorescent protein (GFP) have been used with confocal microscopy and other light microscopic techniques to achieve outstanding temporal resolution, but good spatial and temporal resolution have proven to be difficult to achieve simultaneously. This has been accomplished in a remarkable study by Guido Gaietta, Thomas Deerinck, Stephen Adams, James Bouwer, Oded Tour, Dale Laird, Gina Sosinsky, Roger Tsien, and Mark Ellisman, who demonstrated a pulse-chase technique that correlates with both fluorescence and electron microscopy.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2003

References

2 Gaietta, G., Deerinck, T.J., Adams, S.R., Bouwer, J., Tour, O., Laird, D.W., Sosinsky, G,E., Tsien, R.Y., and Ellisman, M.H., Multicolor and Electron Microsc. Imaging of Connexin Trafficking, Science 296:503-507, 2002.Google Scholar