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The NSOM Technique And It's Significance

Published online by Cambridge University Press:  14 March 2018

Gary Aden*
Affiliation:
TopoMetrix Corporation

Extract

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Prior to Near-Field Scanning Optical Microscopy (NSOM) there were two major light microscopy techniques; optical and confocal.

In an optical microscope a sample is illuminated with a flood of light. The lighted area is then imaged and magnified by collecting the light that is either reflected from or transmitted through the sample by a series of glass lenses. A color magnified image of the sample may be seen directly or displayed on a TV screen. Even if the lenses could be made perfectly, the resolution and magnification of an optical microscope are limited by diffraction effects to approximately one half of the wavelength of the light that is used. Optical microscopes are used routinely to image the general shape of samples as small as human chromosomes or compact disk bits.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 1994