Immunofluorescence techniques using multicolor labeling and analysis of colocalization are essential for unraveling the structural and functional complexity of cells and tissues. Improvements in the capabilities of these techniques are dependent on the development of new fluorescent dyes and methods for attaching them to antibodies and other markers.

The Alexa Fluor® series of dyes developed by researchers at Molecular Probes, Inc. have optimized spectroscopic and physical properties that enable preparation of bioconjugates with consistently strong and photostable fluorescence output.