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Confocal Laser Microscopy on Biofilms: Successes and Limitations

Published online by Cambridge University Press:  14 March 2018

Betsey Pitts  and
Philip Stewart
Betsey Pitts*
Affiliation:
Center for Biofilm Engineering, Montana State University, Bozeman, Montana
Philip Stewart
Affiliation:
Center for Biofilm Engineering, Montana State University, Bozeman, Montana
*
betsey_p@erc.montana.edu

Extract

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Biofilms are communities of microbial cells and extracellular polymeric substances (EPS) attached to a surface. Bacteria exist preferentially in the biofilm state in nature and in many engineered systems. In this state, microbes tolerate antibiotics and disinfectants. Bacterial biofilms are now implicated in a wide range of human diseases and infections including cystic fibrosis, otitis media, chronic wounds, artificial joint and prosthesis infection, and many nosocomial infections. Biofilm-related infections and industrial fouling problems cost billions of dollars each year.

Type
Research Article
Information
Microscopy Today , Volume 16 , Issue 4 , July 2008 , pp. 18 - 23
Copyright
Copyright © Microscopy Society of America 2008

References

ASTM E2562-07 Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with High Shear and Continuous Flow using CDC Biofilm ReactorGoogle Scholar
Goeres, D.M., Loetterle, L.R., Hamilton, M.A., Murga, R., Kirby, D.W., and Donlan, R.M. 2005. Statistical assessment of a laboratory method for growing biofilms. Microbiology. 151: 757-762.Google ScholarPubMed
Werner, E., Roe, F., Bugnicourt, A., Franklin, M.J., Heydorn, A., Molin, S., Pitts, B., and Stewart, P.S., “Stratified Growth in Pseudomonas aeruginosa Biofilms,” Appl. Environ. Microbiol., 70(10):6188-6196 (2004)Google Scholar
Vlamakis, H., Aguilar, C., Losick, R., Kolter, R., “Control of cell fate by the formation of an architecturally complex bacterial community,” Genes Dev. 2008 Apr 1;22(7):945-53.Google Scholar