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Automated, Robotic Preparation of Vitrified Samples for 2D and 3D Cryo Electron Microscopy

Published online by Cambridge University Press:  14 March 2018

Peter. M. Frederik  and
Marc M.H. Storms
Peter. M. Frederik*
Affiliation:
University Maastricht, EM-unit, Universiteitssingel 50, 6229 ER Maastricht, The Netherlands
Marc M.H. Storms*
Affiliation:
FEI Company, Achtseweg Noord 5, 5600 KA Eindhoven, The Netherlands
*
peter.frederik@elmi.unimaas.nl
marc.storms@nl.feico.com

Extract

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Fundamental research within the scope of cell and structural biology as well as nano-technology is increasingly focusing on unraveling interactive biological and biochemical processes and pathways at the macromolecular level. For this, high resolution transmission electron microscopy (TEM) is indispensable. Of paramount importance is the three-dimensional visualization of macromolecular structures and molecular machines in their native hydrated state. Their physical fixation within ultra-thin vitrified ice layers is the crucial starting point.

Type
Research Article
Information
Microscopy Today , Volume 13 , Issue 6 , November 2005 , pp. 32 - 39
Copyright
Copyright © Microscopy Society of America 2005

References

1 Dubochet, J., Adrian, M., Chang, J.J., Homo, J.C., Lepault, J., McDowall, A.W. and Schultz, P. (1992). Cryo-electron microscopy of vitrified specimens. Q.Rev. Biophys. 21, 129–228 Google Scholar
2 Frederik, P.M. and Hubert, D.H.W. (2005). Cryo-electron microscopy of liposomes. Methods in Enzymology vol 391, 431–448.Google Scholar
3 Butter, K, Bomans, P.H.H., Frederik, P.M., Vroege, G.J. and Philipse, A.P. (2003). Direct observation of dipolar chains in iron ferrofluids by cryogenic electron microscopy. Nature Materials 2, 88–91.Google Scholar
4 Carragher, et al., (2004) Journal of Synchrotron Radiation 11:83–85.Google Scholar
5 Lasic, D.D., Ceh, B., Stuart, M.C., Guo, L., Frederik, P.M. and Barenholz, Y. (1995). Transmembrane gradient driven phase transitions within vesicles: Lessons for Drug Delivery. BioChim. Biophys. Acta 1239, 145–156.Google Scholar
6 Elands, J. and Hax, W.H.A. (2004). Current Drug Discovery, 15–20.Google Scholar