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Ultrastable Gold Substrates Improve the Resolution of 3D Reconstructed Density Maps from Electron Micrographs and Tomograms

Published online by Cambridge University Press:  25 July 2016

Christopher J. Russo ,
Israel S. Fernandez ,
Tanmay A. M. Bharat  and
Lori A. Passmore
Christopher J. Russo
Affiliation:
Medical Research Council Laboratory of Molecular Biology, CambridgeUnited Kingdom
Israel S. Fernandez
Affiliation:
Medical Research Council Laboratory of Molecular Biology, CambridgeUnited Kingdom
Tanmay A. M. Bharat
Affiliation:
Medical Research Council Laboratory of Molecular Biology, CambridgeUnited Kingdom
Lori A. Passmore
Affiliation:
Medical Research Council Laboratory of Molecular Biology, CambridgeUnited Kingdom

Abstract

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Abstract
Information
Microscopy and Microanalysis , Volume 22 , Supplement S3: Proceedings of Microscopy & Microanalysis 2016 , July 2016 , pp. 1148 - 1149
Copyright
© Microscopy Society of America 2016 

References

References:

[1] Russo, C. J. & Passmore, L.A. Science 346, 1377–1380, 2014.CrossRefGoogle Scholar
[2] Henderson, R. & McMullan, G. Microscopy 62, 43–50, 2013.CrossRefGoogle Scholar
[3] Bai, ■., et al., eLife 2, e00416 (2013).Google Scholar
[4] Böttcher, , et al., Nature 386, 88–91, 1997.Google Scholar
[5] The authors thank S. Scotcher, A. Fowle, J. Firman, M. Kyte and H. Andrews for fabrication of custom machines; K. Vinothkumar for hepatitis B specimen; J. Grimmett, T. Darling, G. McMullan, S. Chen and C. Saava for technical assistance, J. Löwe, S. Scheres, R.A. Crowther, and R. Henderson for helpful discussions. This work was supported by a Leverhulme Trust Early Career Fellowship to C. J. Russo, the European Research Council (FP7/2007-2013)/ERC grant agreement no 261151, MRC (UK) grant U105192715 (L.A.P.) and U105184332 (V. Ramakrishnan), a Wellcome Trust Senior Investigator award to V. Ramakrishnan (WT096570); T. A. M. Bharat was supported by FEBS and EMBO (ALTF 3-2013) long-term fellowships.Google Scholar