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Towards Artifact-free FIB-SEM Datasets: Strategies Before, During, and After Image Acquisition

Published online by Cambridge University Press:  30 July 2020

Kedar Narayan ,
Alexandre Laquerre  and
Michael Phaneuf
Kedar Narayan
Affiliation:
National Cancer Institute, NIH & Frederick National Laboratory, Frederick, Maryland, United States
Alexandre Laquerre
Affiliation:
Fibics Inc, Ottawa, Ontario, Canada
Michael Phaneuf
Affiliation:
Fibics Inc, Ottawa, Ontario, Canada

Abstract

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Type
3D Scanning Electron Microscopy Imaging of Biological Samples
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 1374 - 1375
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Copyright
Copyright © Microscopy Society of America 2020

References

Narayan, K. and Subramaniam, S. (2015). Focused Ion Beams in Biology. Nature Methods Nov;12(11):1021-3110.1038/nmeth.3623CrossRefGoogle ScholarPubMed
Xu, C. S. et al. (2017). Enhanced FIB-SEM systems for large-volume 3D imaging. Elife May 13;6Google Scholar
Narayan, K. et al. (2014). Multi-resolution correlative focused ion beam scanning electron microscopy: applications to cell biology. Journal of Structural Biology. Mar 185(3):278-8410.1016/j.jsb.2013.11.008CrossRefGoogle ScholarPubMed
Phaneuf, M. W. and Lagarec, K. G. (2017). Microscopy imaging method and system US9812290B2 filed 31 Jan 2017 & issued 07 Nov 2017.Google Scholar