Hostname: page-component-77c89778f8-gvh9x Total loading time: 0 Render date: 2024-07-21T15:33:32.024Z Has data issue: false hasContentIssue false
  • English
  • Français

Through-grid wicking enables high-speed cryoEM specimen preparation

Published online by Cambridge University Press:  30 July 2021

Yong Zi Tan  and
John Rubinstein
Yong Zi Tan
Affiliation:
The Hospital for Sick Children, United States
John Rubinstein
Affiliation:
The Hospital for Sick Children, Canada

Abstract

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 526 - 528
Check for updates
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Glaeser, R.M., 2021. Preparing Better Samples for Cryo–Electron Microscopy: Biochemical Challenges Do not End with Isolation and Purification. Annual Review of Biochemistry, 90.CrossRefGoogle Scholar
Tan, Y.Z. and Rubinstein, J.L., 2020. Through-grid wicking enables high-speed cryoEM specimen preparation. Acta Crystallographica Section D: Structural Biology, 76(11).Google ScholarPubMed
Rubinstein, J.L., Guo, H., Ripstein, Z.A., Haydaroglu, A., Au, A., Yip, C.M., Di Trani, J.M., Benlekbir, S. and Kwok, T., 2019. Shake-it-off: a simple ultrasonic cryo-EM specimen-preparation device. Acta Crystallographica Section D: Structural Biology, 75(12), pp. 1063-1070.CrossRefGoogle ScholarPubMed
Guo, H., Suzuki, T. and Rubinstein, J.L., 2019. Structure of a bacterial ATP synthase. Elife, 8, p. e43128.CrossRefGoogle ScholarPubMed
Guo, H., Courbon, G.M., Bueler, S.A., Mai, J., Liu, J. and Rubinstein, J.L., 2021. Structure of mycobacterial ATP synthase bound to the tuberculosis drug bedaquiline. Nature, 589(7840), pp. 143-147.CrossRefGoogle Scholar
Noble, A.J., Wei, H., Dandey, V.P., Zhang, Z., Tan, Y.Z., Potter, C.S. and Carragher, B., 2018. Reducing effects of particle adsorption to the air–water interface in cryo-EM. Nature methods, 15(10), pp. 793-795.CrossRefGoogle Scholar
Tan, Y.Z., Baldwin, P.R., Davis, J.H., Williamson, J.R., Potter, C.S., Carragher, B. and Lyumkis, D., 2017. Addressing preferred specimen orientation in single-particle cryo-EM through tilting. Nature methods, 14(8), pp. 793-796.CrossRefGoogle ScholarPubMed