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Three-Dimensional Structure of Isometric Capsids of Bacteriophage T4

Published online by Cambridge University Press:  02 July 2020

T. S. Baker ,
N. H. Olson ,
M. Gingery  and
F. A. Eiserling
T. S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
N. H. Olson
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
M. Gingery
Affiliation:
Department of Microbiology & Molecular Genetics, University of California, Los Angeles, CA90095-1489
F. A. Eiserling
Affiliation:
Department of Microbiology & Molecular Genetics, University of California, Los Angeles, CA90095-1489
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Extract

Coliphage T4 is a large, double-stranded DNA virus (family Myoviridae) which infects Escherichia coli. It is the classic example of a contractile-tailed phage and a wealth of information on viral morphogenesis processes have been obtained on it. Extensive studies of T4 by negative-stain and metal-shadow electron microscopy have shown that mature virions, which contain over 40 different polypeptides, have a prolate capsid that encapsidates the genome (∼175 × 106Da), and several other structural components, including the collar with whiskers, neck, a contractile sheath, and a baseplate with fibers. A complete understanding of processes such as assembly of the viral capsids will require atomic-scale knowledge of the various protein interactions. Details of the process can be extrapolated from knowledge of the initial, transitional, and final states of a structure.

We have initiated an examination of T4 head morphogenesis by studying mutant, isometric capsids ('isometrics’) using cryo-electron microscopy and three-dimensional (3D) image reconstruction methods as applied to particles with icosahedral symmetry.

Type
Electron Cryomicroscopy of Macromolecules
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 254 - 255
Copyright
Copyright © Microscopy Society of America

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References

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9. We thank R. Ashmore for programming. Research supported in part by a grant from the NIH (GM33050) and a shared instrumentation grant from NSF (BIR 9112921) to T.S.B., and research funds from the University of California to F.A.E.Google Scholar