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Thermo Scientific™ Glacios Cryo-TEM: A Versatile 200 kV Tool for Structure-Based Drug Discovery

Published online by Cambridge University Press:  30 July 2021

Ieva Drulyte ,
Xin Zhang ,
Rachel Johnson ,
Adrian Koh ,
Simonas Masiulis ,
Sebastian Unger ,
Evgeniya Pechnikova ,
Denise Wootten ,
Patrick Sexton  and
Matthew Belousoff
Ieva Drulyte
Affiliation:
Thermo Fisher Scientific, Eindhoven, Noord-Brabant, Netherlands
Xin Zhang
Affiliation:
Monash University, United States
Rachel Johnson
Affiliation:
Monash University, United States
Adrian Koh
Affiliation:
Thermo Fisher Scientific, Noord-Brabant, Netherlands
Simonas Masiulis
Affiliation:
Thermo Fisher Scientific, Eindhoven, Netherlands
Sebastian Unger
Affiliation:
Thermo Fisher Scientific, United States
Evgeniya Pechnikova
Affiliation:
Thermo Fisher Scientific, United States
Denise Wootten
Affiliation:
Monash University, United States
Patrick Sexton
Affiliation:
Monash University, United States
Matthew Belousoff
Affiliation:
Monash University, United States

Abstract

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Type
Cryo-EM in Drug Discovery
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 3256 - 3258
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Li, X., Mooney, P., Zheng, S., Booth, C.R., Braunfeld, M.B., Gubbens, S., Agard, D.A. and Cheng, Y., 2013. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nature methods, 10(6), pp.584-590.CrossRefGoogle ScholarPubMed
Bai, X.C., 2021. Seeing Atoms by Single-Particle Cryo-EM. Trends in biochemical sciences.Google Scholar
García-Nafría, J. and Tate, C.G., 2020. Cryo-electron microscopy: Moving beyond X-ray crystal structures for drug receptors and drug development. Annual review of pharmacology and toxicology, 60, pp.51-71.Google ScholarPubMed
De Graaf, C., Donnelly, D., Wootten, D., Lau, J., Sexton, P.M., Miller, L.J., Ahn, J.M., Liao, J., Fletcher, M.M., Yang, D. and Brown, A.J., 2016. Glucagon-like peptide-1 and its class BG protein–coupled receptors: a long march to therapeutic successes. Pharmacological reviews, 68(4), pp.954-1013.CrossRefGoogle Scholar
Zhang, X., Belousoff, M.J., Zhao, P., Kooistra, A.J., Truong, T.T., Ang, S.Y., Underwood, C.R., Egebjerg, T., Šenel, P., Stewart, G.D. and Liang, Y.L., 2020. Differential GLP-1R binding and activation by peptide and non-peptide agonists. Molecular Cell, 80(3), pp.485-500.CrossRefGoogle ScholarPubMed
Zhang, X., Johnson, R.M., Drulyte, I., Yu, L., Kotecha, A., Danev, R., Wootten, D., Sexton, P.M. and Belousoff, M.J., 2021. Evolving cryo-EM structural approaches for GPCR drug discovery. bioRxiv.Google Scholar