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Structural and Ultrastructural Changes in the Tongue of mdx Mice

Part of: Micrographia Collection

Published online by Cambridge University Press:  24 January 2022

André N. Tomiate Open the ORCID record for André N. Tomiate [Opens in a new window] ,
Gabriela K. Barbosa Open the ORCID record for Gabriela K. Barbosa [Opens in a new window] ,
Rose E. G. Rici Open the ORCID record for Rose E. G. Rici [Opens in a new window] ,
Sonia Regina Y. de Almeida ,
Ii-sei Watanabe Open the ORCID record for Ii-sei Watanabe [Opens in a new window]  and
Adriano P. Ciena Open the ORCID record for Adriano P. Ciena [Opens in a new window]
André N. Tomiate
Affiliation:
Laboratório de Morfologia e Atividade Física (LAMAF), Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, São Paulo, Brasil
Gabriela K. Barbosa
Affiliation:
Laboratório de Morfologia e Atividade Física (LAMAF), Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, São Paulo, Brasil
Rose E. G. Rici
Affiliation:
Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil
Sonia Regina Y. de Almeida
Affiliation:
Departamento de Anatomia, Instituto de Ciências Biomédicas - ICB III, Universidade de São Paulo, São Paulo, Brasil
Ii-sei Watanabe
Affiliation:
Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil Departamento de Anatomia, Instituto de Ciências Biomédicas - ICB III, Universidade de São Paulo, São Paulo, Brasil
Adriano P. Ciena*
Affiliation:
Laboratório de Morfologia e Atividade Física (LAMAF), Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, São Paulo, Brasil
*
*Corresponding author: Adriano P. Ciena, E-mail: adriano.ciena@unesp.br
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Abstract

The mdx mouse is an experimental model of Duchenne muscular dystrophy, a genetic disorder characterized by progressive muscular degeneration which affects the oral cavity musculature, and promotes difficulty in swallowing. This study aimed to describe morphological, structural, and ultrastructural changes in the tongue mucosa and musculature of mdx mice. Forty six-month-old mice were divided into two groups: Control C57bl/10 (n = 20) and mdx C57bl/10mdx (n = 20). The tongue was dissected and analyzed with light microscopy, scanning electron microscopy, and transmission electron microscopy techniques. Our results showed conical and triangular filiform, fungiform, foliate, and vallate papillae, and their connective tissue cores. The epithelium layers identified were corneum, granulosum, spinosum, and basale. The mdx group had a thicker epithelium. Lamina propria was composed of reddish and greenish collagen. In mdx, collagen was present in the musculature of the tongue's body and in the muscular tissue between mucous and serous glands of the caudal region. Musculature was also characterized by a shorter length of sarcoplasmic invaginations, myocytolysis in mitochondrial groupings, and inflammatory focus. In conclusion, the tongue of 6-month-old mdx mice had morphology, structure, and ultrastructure revealed, showing higher wear of filiform papillae indirect reflex from the muscular degeneration process.

Keywords

animal modelconnective tissue coresDuchenne muscular dystrophylingual papillaemuscular degenerationrodents
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 28 , Issue 2 , April 2022 , pp. 559 - 566
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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