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Spatial Distribution of Heterochromatin Bodies in the Nuclei of Triatoma infestans (Klug)

Part of: Micrographia Collection

Published online by Cambridge University Press:  12 May 2020

Carlos Henrique L. Imperador ,
Vanessa B. Bardella ,
Eli Heber M. dos Anjos ,
Vera L.C.C. Rodrigues ,
Diogo C. Cabral-de-Mello  and
Maria Luiza S. Mello Open the ORCID record for Maria Luiza S. Mello [Opens in a new window]
Carlos Henrique L. Imperador
Affiliation:
Department of Structural and Functional Biology, Institute of Biology, University of Campinas (Unicamp), Rua Monteiro Lobato 255, 13083-862Campinas, SP, Brazil
Vanessa B. Bardella
Affiliation:
Department of Biology, Institute of Biosciences, State University of São Paulo (Unesp), Avenida 24-A, 1515, 13506-900Rio Claro, SP, Brazil
Eli Heber M. dos Anjos
Affiliation:
Department of Structural and Functional Biology, Institute of Biology, University of Campinas (Unicamp), Rua Monteiro Lobato 255, 13083-862Campinas, SP, Brazil
Vera L.C.C. Rodrigues
Affiliation:
Superintendence for Control of Endemic Diseases (SUCEN), Rua Afonso Pessini, 86, 13845-206Mogi-Guaçu, SP, Brazil
Diogo C. Cabral-de-Mello
Affiliation:
Department of Biology, Institute of Biosciences, State University of São Paulo (Unesp), Avenida 24-A, 1515, 13506-900Rio Claro, SP, Brazil
Maria Luiza S. Mello*
Affiliation:
Department of Structural and Functional Biology, Institute of Biology, University of Campinas (Unicamp), Rua Monteiro Lobato 255, 13083-862Campinas, SP, Brazil
*
*Author for correspondence: Maria Luiza S. Mello, E-mail: mlsmello@unicamp.br
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Abstract

Constitutive heterochromatin typically exhibits low gene density and is commonly found adjacent or close to the nuclear periphery, in contrast to transcriptionally active genes concentrated in the innermost nuclear region. In Triatoma infestans cells, conspicuous constitutive heterochromatin forms deeply stained structures named chromocenters. However, to the best of our knowledge, no information exists regarding whether these chromocenters acquire a precise topology in the cell nuclei or whether their 18S rDNA, which is important for ribosome function, faces the nuclear center preferentially. In this work, the spatial distribution of fluorescent Feulgen-stained chromocenters and the distribution of their 18S rDNA was analyzed in Malpighian tubule cells of T. infestans using confocal microscopy. The chromocenters were shown to be spatially positioned relatively close to the nuclear periphery, though not adjacent to it. The variable distance between the chromocenters and the nuclear periphery suggests mobility of these bodies within the cell nuclei. The distribution of 18S rDNA at the edge of the chromocenters was not found to face the nuclear interior exclusively. Because the genome regions containing 18S rDNA in the chromocenters also face the nuclear periphery, the proximity of the chromocenters to this nuclear region is not assumed to be associated with overall gene silencing.

Keywords

chromatin topologychromocenterconfocal microscopyFISHheterochromatin18S rDNA
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 26 , Issue 3 , June 2020 , pp. 567 - 574
Copyright
Copyright © Microscopy Society of America 2020

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