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Secretion of Brain Natriuretic Peptide in Cultured Human Coronary Vascular Smooth Muscle Cells

Published online by Cambridge University Press:  02 July 2020

J. Lin  and
C. Wei
J. Lin
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery and Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201
C. Wei
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery and Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201
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Extract

Brain natriuretic peptide (BNP) is a peptide of cardiac origin which regulates plasma volume as well as vascular tone and growth. Recently, we have reported that brain natriuretic peptide is a potent inhibitor of endothelin-1-mediated proliferation in human coronary vascular smooth muscle cells (HCoVSMC). While brain natriuretic peptide has been reported to be produced and released from atrial and ventricular myocardium, we hypothesize that brain natriuretic peptide may be present and secreted from human coronary vascular smooth muscle cells.

Therefore, the present study was designed to investigate the secretion of brain natriuretic peptide in cultured human coronary vascular smooth muscle cells (HCoVSMC: Clonetics, San Diego, CA). The concentration of brain natriuretic peptide, and its second messenger cGMP, in culture media (48 hours) was determined by radioimmunoassay (Phoenix, Mountain View, CA). The presence of brain natriuretic peptide was determined by immunohistochemical staining using a human brain natriuretic peptide polyclonal antibody.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 598 - 599
Copyright
Copyright © Microscopy Society of America

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References

References:

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5. This research was supported in part by grants from the NIH (HL03174 & HL61299, C. Wei), AHA-MD, NKF and University of Maryland School of Medicine.Google Scholar