Hostname: page-component-5c6d5d7d68-qks25 Total loading time: 0 Render date: 2024-08-18T16:16:03.116Z Has data issue: false hasContentIssue false
  • English
  • Français

Quantitative Elemental Analysis of the Components of Pb Exposed Cells Of Plectonema Boryanum Using Regular and Overplus Cells: an Energy Disspersive X-Ray Spectroscopy Study

Published online by Cambridge University Press:  02 July 2020

J.J. Goldberg  and
T.E. Jensen
J.J. Goldberg
Affiliation:
Department of Biological Sciences, Lehman College & CUNY Grad. Center, Bronx, NY, 10468
T.E. Jensen
Affiliation:
Department of Biological Sciences, Lehman College & CUNY Grad. Center, Bronx, NY, 10468
Get access

Extract

The elemental composition of polyphosphate bodies (PPB’s) and other components of the cell have previously been determined by using energy dispersive X-ray spectrometers(EDX). In this present study we perform a quantitative analysis or normally grown cells and cells that were grown in the overplus phenomenon.

Cells of Plectonema boryanum were grown in modified Fitzgerald's media and harvested after a logarithmic growth phase of 14 days. The overplus cells were grown as has been previously described2. The cells were exposed to l00ppm of lead, as well as other metals separately, for one hour. The cells were then fixed and embedded in EPON according to Luft's procedure.

For X-ray analysis cells were first located using the TEM mode, and then the microscope was switched to the STEM mode. Analysis of cell components were carried out using the spot mode (75Kv).

Type
New Trends in Scanning Electron Microscopy and Microanalysis
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 262 - 263
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Jensen, T.E. et.al, Environ Pollut Ser A 27(1982)CrossRefGoogle Scholar
2.Baxter, M. and Jensen, T.E., Cytobios 45 (1986) 147Google Scholar
3.Luft, J.H., J Biophys Biochem Cytol 9(1961) 409CrossRefGoogle Scholar
4.Hall, T.A., J Microscopie Biol. Cell 22 (1975) 271Google Scholar
5.Heldal, M., Applied and Environmental MicrobiologyGoogle Scholar
6.Pankratz, H.S. and Bowen, C.C., Am J Bot 50(1963)387CrossRefGoogle Scholar
7.Goldberg, J. et.al., Microscopy and Microanalysis 1996 802CrossRefGoogle Scholar