Hostname: page-component-84b7d79bbc-fnpn6 Total loading time: 0 Render date: 2024-07-27T22:29:58.770Z Has data issue: false hasContentIssue false
  • English
  • Français

Presence of Caveolae in the Granular Epithelial Cells of Rabbit Urinary Bladder

Published online by Cambridge University Press:  02 July 2020

A.J. Mia ,
L.X. Oakford  and
T. Yorio
A.J. Mia
Affiliation:
Jarvis Christian College, Highway 80, Hawkins, TX, 75765
L.X. Oakford
Affiliation:
University of North Texas Health Science Center at Fort Worth, TX, 76107
T. Yorio
Affiliation:
University of North Texas Health Science Center at Fort Worth, TX, 76107
Get access

Extract

Previously, we reported the presence of caveolae, clathrin-coated pits and vesicles in toad urinary bladder granular epithelial cells, and in smooth muscle and endothelial cells. Since then we investigated the occurrence of caveolae in the rabbit urinary bladder cells. Here we report for the first time the presence of caveolae and clathrin-coated pits and vesicles in the granular epithelial, smooth muscle and endothelial cells of rabbit urinary bladders.

Urinary bladder sacs were surgically removed from doubly pithed toads and anaesthetized rabbits, and allowed fixation for 1 hr in 2% glutaraldehyde in PIPES buffer. These bladder tissues were divided for scanning (SEM) and transmission electron microscopy (TEM) for comparative analysis. A postfixation using 1% osmium tetroxide was carried out for 1 hr. Tissues were appropriately processed for SEM and TEM observations. Ultrathin sections, made with a diamond knife, were taken on bare nickel grids and then exposed to uranyl acetate and lead citrate for viewing in the TEM.

Type
Imaging Cells and Organelles
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 253 - 254
Copyright
Copyright © Microscopy Society of America 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Mia, A. J. et al., FASEB J. 10(1996)161.Google Scholar
2.Mia, A. J. et al., Proc. MSA (1996)934–935.Google Scholar
3.Mia, A. J. et al., Tissue & Cell, 26(1994)189.10.1016/0040-8166(94)90094-9CrossRefGoogle Scholar
4.Rothberg, K. G. et al., Cell, 68(1992)673.10.1016/0092-8674(92)90143-ZCrossRefGoogle Scholar
5.Stang, E. et al., Mol. Biol. Cell, 8(1997)47.10.1091/mbc.8.1.47CrossRefGoogle Scholar
6. Supported in part by grant DAMD17-95-5086.Google Scholar