Vascular corrosion replicas provide unique three-dimensional models of both macro- and microcirculatory angio-architecture. In most replication procedures, blood is initially flushed from the vasculature with a low viscosity, non-clotting medium, such as saline, prior to infusion with the unpolymerized resin. While this removes cellular elements from the vessels and prevents coagulation, the chemical characteristics of the saline and the mode in which it is infused can potentially affect vascular smooth muscle. This can be disadvantageous if subsequent distribution of the resin does not faithfully mimic normal perfusion pathways, or if the vascular lumen is either narrowed due to vasospasm or distended due to excessive intravascular pressure. Alternatively, the composition of the saline can be modified to enhance the replication process. This may be done merely to ensure complete filling of the vasculature or to evaluate anatomical foci instrumental in physiological and pharmacological regulation of perfusion distribution.