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Optimization of the Excitation Light Sheet in Selective Plane Illumination Microscopy

Published online by Cambridge University Press:  23 September 2015

Liang Gao*
Affiliation:
Department of Chemistry, Stony Brook University, Stony Brook, NY, USA Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA

Abstract

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Type
Abstract
Copyright
Copyright © Microscopy Society of America 2015 

References

[1] Huisken, J., et al, Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy. Science 305, 10071009 (2004).Google Scholar
[2] Keller, P. J., et al, Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Science 322, 10651069 (2008).Google Scholar
[3] Krzic, U., et al, Multiview light-sheet microscope for rapid in toto imaging. Nat. Methods 9, 730733 (2012).Google Scholar
[4] Planchon, T. A., et al, Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination. Nat. Methods 8, 417423 (2011).Google Scholar
[5] Tomer, R., et al, Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy. Nat. Methods 9, 755763 (2012).Google Scholar
[6] Wu, Y., et al, Inverted selective plane illumination microscopy (iSPIM) enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U.S.A 108, 1770817713 (2011).Google Scholar
[7] Gao, L., et al, 3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy. Nat. Protocols 9, 1083–1011 (2014).Google Scholar
[8] Gao, L., et al, Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens. Cell 151, 13701385 (2012).Google Scholar
[9] Vettenburg, T., et al, Light-sheet microscopy using an Airy beam. Nat. Methods 11, 541544 (2014).Google Scholar
[10] Chen, B.-C., et al, Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. Science 346, 1257998 (2014).Google Scholar