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Nucleologenesis in Trypanosoma cruzi

Published online by Cambridge University Press:  29 April 2016

Tomás Nepomuceno-Mejía ,
Reyna Lara-Martínez ,
Roberto Hernández ,
María de Lourdes Segura-Valdez  and
Luis F. Jiménez-García
Tomás Nepomuceno-Mejía
Affiliation:
Laboratory of Electron Microscopy, Faculty of Science, National Autonomous University of Mexico, México Cd. Mx. 04510, México
Reyna Lara-Martínez
Affiliation:
Laboratory of Electron Microscopy, Faculty of Science, National Autonomous University of Mexico, México Cd. Mx. 04510, México
Roberto Hernández
Affiliation:
Department of Molecular Biology and Biotechnology, Biomedical Research Institute, National Autonomous University of Mexico, México Cd. Mx. 04510, México
María de Lourdes Segura-Valdez
Affiliation:
Laboratory of Electron Microscopy, Faculty of Science, National Autonomous University of Mexico, México Cd. Mx. 04510, México
Luis F. Jiménez-García*
Affiliation:
Laboratory of Electron Microscopy, Faculty of Science, National Autonomous University of Mexico, México Cd. Mx. 04510, México
*
*Corresponding author. luisfelipe_jimenez@ciencias.unam.mx
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Abstract

Nucleolar assembly is a cellular event that requires the synthesis and processing of ribosomal RNA, in addition to the participation of pre-nucleolar bodies (PNBs) at the end of mitosis. In mammals and plants, nucleolar biogenesis has been described in detail, but in unicellular eukaryotes it is a poorly understood process. In this study, we used light and electron microscopy cytochemical techniques to investigate the distribution of nucleolar components in the pathway of nucleolus rebuilding during closed cell division in epimastigotes of Trypanosoma cruzi, the etiologic agent of American trypanosomiasis. Silver impregnation specific for nucleolar organizer regions and an ethylenediaminetetraacetic acid regressive procedure to preferentially stain ribonucleoprotein revealed the conservation and dispersion of nucleolar material throughout the nucleoplasm during cell division. Furthermore, at the end of mitosis, the argyrophilic proteins were concentrated in the nucleolar organizer region. Unexpectedly, accumulation of nucleolar material in the form of PNBs was not visualized. We suggest that formation of the nucleolus in epimastigotes of T. cruzi occurs by a process that does not require the concentration of nucleolar material within intermediate nuclear bodies such as mammalian and plant PNBs.

Keywords

Trypanosoma cruzinucleolusnucleolar assemblyclosed mitosispre-nucleolar bodies
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 3 , June 2016 , pp. 621 - 629
Copyright
Copyright © Microscopy Society of America 2016

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Footnotes

†

Current address: CONACYT Research Fellow, Regional Center for Public Health Research, Tapachula, Chiapas, México.

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