Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-07-02T04:08:40.251Z Has data issue: false hasContentIssue false

Modification of an Energy Filtering Transmission Electron Microscope for Low Energy Loss Imaging and Chromophore Mapping

Published online by Cambridge University Press:  02 July 2020

J. A. Davis
Affiliation:
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, ONM5G2M9, Canada
Y. M. Heng
Affiliation:
Electron Microscopy Facility, McMaster University, Hamilton, ONL8N3Z5, Canada
M. M. G. Barfels
Affiliation:
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, ONM5G2M9, Canada
F. P. Ottensmeyerf
Affiliation:
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, ONM5G2M9, Canada
Get access

Extract

We have recently replaced the electron energy filter of the Zeiss EM 902 with an improved PMP filter. The design, incorporating concave curved entrance and exit faces and convex curves on the side facing the electrostatic mirror was based on the theoretical calculations of Jiang and Ottensmeyer and Barfels et al. Care was taken in the design of the filter to allow its introduction into the existing geometry of the microscope and its use of the existing electronics. As a result, dismantling the existing prism housing and support (which were used as the base for the new prism), reinstallation, and adjustment required only three working days. The resolution in tjie energy dispersive plane of the new filter was measured using the 15.0 eV plasmon peak of aluminum as an internal standard. Such measurements yielded a FWHM of 1.1 eV over the full image plane (fig. 1) with an optimum resolution of approximately 0.8 eV possible with corrections to the stability of the voltage supply and further small alignment adjustments.

This improved energy resolution permits the detection and imaging of signals in the low loss region previously inaccessible to us in the EM902.

Type
Advances in Instrumentation
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Jiang, X. G. and Ottensmeyer, F. P., Optik 94(1993)88Google Scholar
2.Barfels, M. M. G.et al., Micron 29(1998)97CrossRefGoogle Scholar
3.Egerton, R. F., Electron Energy-Loss Spectroscopy in the Electron Microscope, Plenum Press (1996)157CrossRefGoogle Scholar