Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-21T22:05:36.690Z Has data issue: false hasContentIssue false
  • English
  • Français

Leaf Anatomy and Histochemistry of Three Species of Ficus sect. Americanae Supported by Light and Electron Microscopy

Published online by Cambridge University Press:  11 November 2013

Nathalia Diniz Araújo ,
Victor Peçanha M. Coelho ,
Marília Contin Ventrella  and
Maria de Fátima Agra
Nathalia Diniz Araújo
Affiliation:
Laboratório de Farmacobotânica, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, 58051-970, João Pessoa, Paraíba, Brazil
Victor Peçanha M. Coelho
Affiliation:
Laboratório de Anatomia Vegetal, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
Marília Contin Ventrella
Affiliation:
Laboratório de Anatomia Vegetal, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
Maria de Fátima Agra*
Affiliation:
Laboratório de Farmacobotânica, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, 58051-970, João Pessoa, Paraíba, Brazil
*
*Corresponding author. E-mail: agramf@ltf.ufpb.br
Get access

Abstract

In this work the leaf anatomy of three species of Ficus section Americanae (Miq.) Miq. from Brazil, whose leaves and latex are used in folk medicine is reported. The work was carried out using light and scanning electron microscopy in order to characterize these species and to evaluate their taxonomic significance, and also contribute to the quality control of their ethnodrugs. The three species (Ficus cyclophylla, Ficus elliotiana, and Ficus caatingae) showed hypostomatic leaves, anomocytic stomata, straight epidermal cell outlines, and a dorsiventral mesophyll. Some micro-morphological characters such as density and distribution of epicuticular waxes, glandular trichomes, the length and width of stomata, as well as the palisade of mesophyll and petiole outlines proved to be the most useful and distinctive characters for the separation of species. These may contribute as additional support for the taxonomy of the section and for the quality control of their ethnodrugs.

Keywords

Ficus subg. UrostigmaMoraceaeSEMmedicinal plantsFicus cyclophyllaFicus elliotianaFicus caatingae
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 1 , February 2014 , pp. 296 - 304
Copyright
Copyright © Microscopy Society of America 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aguiar, T.V., Sant'anna-Santos, B.F., Azevedo, A.A. & Ferreira, R.S. (2007). ANATI QUANTI: Software de análises quantitativas para estudos em anatomia vegetal. Plant Daninha 25, 649–659.Google Scholar
Barthlott, W. (1981). Epidermal and seed surface characters of plants: Systematic applicability and some evolutionary aspects. Nord J Bot 1, 345–355.Google Scholar
Barthlott, W., Neinhuis, C., Cutler, D., Ditsch, F., Meusel, I., Theisen, I. & Wilhelmi, H. (1998). Classification and terminology of plant epicuticular waxes. J Linn Soc Bot 126, 237–260.Google Scholar
Berg, C.C. (1989). Systematics and phylogeny of the Urticales. In Evolution, Systematics, and Fossil History of the Hamamelidae 2, ‘Higher’ Hamamelidade, Crane, P.R. & Blackmore, S. (Eds.), pp. 193–220. Oxford, UK: Clarendon Press.Google Scholar
Berg, C.C. (2007). Proposals for treating four species complexes in Ficus subgenus Urostigma section Americanae (Moraceae). Blumea 52, 295–312.Google Scholar
Brundrett, M.C., Kendrick, B. & Peterson, C.A. (1991). Efficient lipid staining in plant material with Sudan Red 7B or Fluoral Yellow 088 in polyethylene glycerol-glycol. Biotechnic & Histochemistry 66, 111–116.Google Scholar
Buvat, R. (1989). Ontogeny, Cell Differentiation, and Structure of Vascular Plants. Berlin, Heidelberg: Springer Verlag.Google Scholar
Carauta, J.P.P. & Diaz, B.E. (2002). Figueiras no Brasil. Rio de Janeiro: Editora UFRJ.Google Scholar
Carvajal, S. & Shabes, L.K. (2005). Estructura anatómica de lós pecíolos de especies americanas del género Ficus L. (Moraceae). Ibugana 13, 49–66.Google Scholar
Castro, R.M. & Rapini, A. (2006). Four new species of Moraceae from Bahia, Brasil. Neodiversity 1, 13–20.Google Scholar
Cronquist, A. (1981). An Integrated System of Classification of Flowering Plants. New York: Columbia University Press.Google Scholar
David, R. & Carde, J.P. (1964). Coloration différentielle dês inclusions lipidique et terpeniques dês pseudophylles du Pin maritime au moyen du reactif Nadi. Comptes Rendus Hebdomadaires dês Séances de l'Academie dês Sciences Paris, D258, pp. 1338–1340.Google Scholar
Forman, L. & Bridson, D. (1989). The Herbarium Handbook. Kew, UK: Royal Botanic Gardens.Google Scholar
Furr, M. & Mahlberg, P.G. (1981). Histochemical analyses of lacticifers and glandular tricomes in Cannabis sativa . J Nat Prod 44, 153–159.Google Scholar
Herre, E.A. (1989). Coevolution of reproductive characteristics in 12 species of New World figs and their pollinator wasps. Experientia 45, 637–647.Google Scholar
Herre, E.A., Machado, C.A., Bermingham, E., Nason, J.D. & Windsor, D.M. (1996). Molecular phylogenies of figs and their pollinator wasps. J Biog 23, 521–530.Google Scholar
Johansen, D.A. (1940). Plant Microtechinique, 3rd ed. New York: Paul B. Hoeber, Inc. Google Scholar
Jousselin, E., Hossaert-Mckey, M., Herre, E.A. & Kjellberg, F. (2003). Why do fig wasps actively pollinate monoecious figs? Oecologia 134, 381–387.Google Scholar
Kim, K.W. (2008). Visualization of micromorphology of leaf epicuticular waxes of the rubber tree Ficus elastica by electron microscopy. Micron 39, 976–984.Google Scholar
Mace, M.E. & Howell, C.R. (1974). Histochemistry and identification of condensed tannin precursor in roots of cotton seedlings. Can J Bot 52, 2423–2426.Google Scholar
Metcalfe, C.R. & Chalk, L. (1950). Anatomy of the Dicotyledons: Leaves, Stem, and Wood in Relation to Taxonomy with Notes on Economic Uses. Oxford, UK: Clarendon Press.Google Scholar
Miquel, F.A.G. (1853). Urticineae. Fl bras 12, 77–222.Google Scholar
Neves, L.J., Mello-Filho, L.E., Fernandes, L.D.R.S. & Ferreira, A.K. (1991). Anatomia foliar de Ficus semicordata B. Ham. Ex J.E. Smith. Bradea 5, 479–496.Google Scholar
O'Brien, T.P., Feder, N. & McCully, M.E. (1964). Polycromatic staining of plant cell walls by toluidine blue O. Protoplasma 59, 368–373.Google Scholar
Ogunkunle, A.T.J. & Oladele, F.A. (2008). Leaf epidermal studies in some Nigerian species of Ficus L. (Moraceae). Plant Syst Evol 274, 209–221.Google Scholar
Pearse, A.G.E. (1980). Histochemistry Theoretical and Applied. Edinburgh, UK: Churchill Livingston.Google Scholar
Porto, N.M., Figueiredo, R.C.B.Q., Oliveira, A.F.M. & Agra, M.F. (2011). Leaf epidermal characteristics of Cissampelos L. (Menispermaceae) species from northeastern Brazil. Microsc Res Tech 74, 370–376.Google Scholar
Quintanar, A., Castrejón, J.L.Z., López, C. & Salgado-Ugarte, I.H. (2004). Anatomía e histoquímica de la corteza de cinco especies de Moraceae. Polibotánica 17, 15–38.Google Scholar
Rønsted, N., Weiblen, G.D., Clement, W., Zerega, N. & Savolainen, V. (2008). Reconstructing the phylogeny of figs (Ficus, Moraceae) to unravel the origin of fig-wasp mutualisms. Symbiosis 45, 45–56.Google Scholar
Solereder, H. (1908). Systematic Anatomy of the Dicotyledons. A Handbook for Laboratories of Pure and Applied Botany. Oxford, UK: Clarendon Press.Google Scholar
Sonibare, M.A., Jayeola, A.A., Egunyomi, A. & Murata, J. (2005). A survey of epidermal morphology in Ficus Linn. (Moraceae) of Nigeria. Bot Bull Acad Sin 46, 231–238.Google Scholar
Van Greuning, J.V., Robbertse, P.J. & Grobbelaar, N. (1984). The taxonomic value of leaf anatomy in the genus Ficus. South African J Bot 3, 297–305.Google Scholar
Verkerke, W. (1989). Structure and function of the fig. Experientia 45, 612–622.Google Scholar
Weiblen, G.D. (2000). Phylogenetic relationships of functionally dioecious Ficus (Moraceae) based on ribosomal DNA sequences and morphology. AJB 87, 1342–1357.Google Scholar
Weiblen, G.D. (2001). Phylogenetic relationships of fig wasps pollinating functionally dioecious Ficus based on mitochondrial DNA sequences and morphology. Syst Biol 50, 243–267.Google Scholar