Hostname: page-component-7479d7b7d-767nl Total loading time: 0 Render date: 2024-07-11T15:21:11.687Z Has data issue: false hasContentIssue false
  • English
  • Français

In Situ TEM Study of DNA/Gold Nanoparticles in Liquid Environment

Published online by Cambridge University Press:  02 July 2020

W.-A Chiou ,
R. C. Mucic ,
A. Ishikawa ,
H. Konishi ,
K. Fukushima  and
C. A. Mirkin
W.-A Chiou
Affiliation:
Department, of Materials Science and Engineering, Northwestern University, Evanston, IL60208
R. C. Mucic
Affiliation:
Department, of Chemistry, Northwestern University, Evanston, IL60208
A. Ishikawa
Affiliation:
Department, of Physics, Nihon University, Sakurajosui, Setagaya-Ku, Tokyo 156, Japan
H. Konishi
Affiliation:
Department, of Physics, Nihon University, Sakurajosui, Setagaya-Ku, Tokyo 156, Japan
K. Fukushima
Affiliation:
JEOL Ltd., 1-2 Musashino, 3-Chome, Akishima, Tokyo 196, Japan
C. A. Mirkin
Affiliation:
Department, of Chemistry, Northwestern University, Evanston, IL60208
Get access

Extract

In recent years the assembly of nanometer sized building blocks into two- and three-dimentional structures and functional materials has been an extremely active area of scientific research. A new class of materials, the DNA/nanoparticle hybrid materials and assemblies, which might have useful electrical, optical and structural properties, have been developed at Northwestern University. While characterizing particle morphology (size and shape) by subjecting it to the high vacuum environment in an electron microscope, the structure may have changed drastically from its natural state. The study of morphology of these DNA/Au nanoparticles in the liquid state was thus undertaken to further our understanding of the dynamics of the nanoparticles in a specific environment. This paper presents an in-situ observation of DNA/Au nanoparticle assembles in liquid media using environmental TEM.

Experiments were carried out with citrate-stabilized colloidal Au particles averaging 8 and 31 nm in diameter. Colloidal Au nanoparticles were obtained from a commercial source. 8 nm particles were modified with propylthiol-capped oligonucleotide, 3’HS(CH2)3-O(O)P(O)O-ATG-CTC-AAC-TCT, 1,whereas the 31 nm particles were modified with hexlylthiol-capped oligonucleotide, 3’TAG-GAC-TTA-CGC-O(O)P(O )O-(CH2)6SH, 2.

Type
In-Situ Microscopy Techniques
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 340 - 341
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Alivisators, A. P., Science, 271 (1996) 933; Bethell, D., Schiffrin, D. J., Nature 382 (1996) 581.CrossRefGoogle Scholar

2. Mirkin, C. A., et. al., Nature, 382 (1996) 607; Mucic, R. C., et. al., J. ACS, 120 (1998) 12674.CrossRefGoogle Scholar

3. Mucic, R. C.Ph.D., Dissertation, Dept. of Chemistry, Northwestern University, 1999.Google Scholar

4. Fukami, A.et. al., EMSA Proceeding, 45 (1987) 142.Google Scholar

5. This research made use of MRC Facilities supported by the NSF (DMR-9632742) at NU.Google Scholar