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Imaging and Volumetric Quantitation of Vascular Corrosion Casts with Laser Scanning Confocal Microscopy.

Published online by Cambridge University Press:  02 July 2020

K. Czymmek ,
R. C. Wagner ,
F. E. Hossler  and
R. Kao
K. Czymmek
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, Delaware, 19716
R. C. Wagner
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, Delaware, 19716
F. E. Hossler
Affiliation:
Department of Anatomy and Cell Biology East Tennessee State University, Quillen College of Medicine, Johnson City, TN, 37614
R. Kao
Affiliation:
Dept. of Surgery East Tennessee State University, Quillen College of Medicine, Johnson City, TN, 37614
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Extract

Vascular corrosion casts provide faithful replicas of the three-dimensional anatomy of blood vessel system of organs and tissues. In addition, corrosion casts can be used to obtain quantitative information regarding vessel and tissue spaces. However, morphometric measurements of SEM images of corrosion casts is difficult due to severe specimen tilt, parallax and the inability to image 3D casts from all angles. We present evidence that confocal microscopy can be used to collect all of the 3D information of a corrosion cast in a z-series of optical slices. Surface and volumetric parameters of casted and non-casted space are readily retrievable from a stack of optical sections through a corrosion cast.

Various tissues and organs were exsanguinated with heparenized saline following cannulization of regional arteries and casts were made by infusing Mercox resin or Mercox diluted with 20% methyl methacrylate monomer. Tissues were macerated with alternating rinses in 5% KOH and hot water.

Type
Applications and Advances in Vascular Corrosion Casting in Microvascular Research
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 562 - 563
Copyright
Copyright © Microscopy Society of America

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References

1. Kratky, R. G., et al., Scann. Microsc. 3:937 (1989)Google Scholar

2. Hossler, F. E., Microsc. & Microanal. 4: (sup.2. Proceed): 914(1998)CrossRefGoogle Scholar